Recombinant Full Length Human ALX4 Protein, GST-tagged

• Cat.No.: ALX4-1250HF
• Product Overview: Human ALX4 full-length ORF ( AAI66622.1, 1 a.a. - 411 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a paired-like homeodomain transcription factor expressed in the mesenchyme of developing bones, limbs, hair, teeth, and mammary tissue. Mutations in this gene cause parietal foramina 2 (PFM2); an autosomal dominant disease characterized by deficient ossification of the parietal bones. Mutations in this gene also cause a form of frontonasal dysplasia with alopecia and hypogonadism; suggesting a role for this gene in craniofacial development, mesenchymal-epithelial communication, and hair follicle development. Deletion of a segment of chromosome 11 containing this gene, del(11)(p11p12), causes Potocki-Shaffer syndrome (PSS); a syndrome characterized by craniofacial anomalies, mental retardation, multiple exostoses, and genital abnormalities in males. In mouse, this gene has been shown to use dual translation initiation sites located 16 codons apart. [provided by RefSeq, Oct 2009]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 45.3 kDa
• Protein Length: 411 amino acids
• AA Sequence: MNAETCVSYC ESPAAAMDAY YSPVSQSREG SSPFRAFPGG DKFGTTFLSA AAKAQGFGDA KSRARYGAGQ QDLATPLESG AGARGSFNKF QPQPSTPQPQ PPPQPQPQQQ QPQPQPPAQP HLYLQRGACK TPPDGSLKLQ EGSSGHSAAL QVPCYAKESS LGEPELPPDS DTVGMDSSYL SVKEAGVKGP QDRASSDLPS PLEKADSESN KGKKRRNRTT FTSYQLEELE KVFQKTHYPD VYAREQLAMR TDLTEARVQV WFQNRRAKWR KRERFGQMQQ VRTHFSTAYE LPLLTRAENY AQIQNPSWLG NNGAASPVPA CVVPCDPVPA CMSPHAHPPG SGASSVTDFL SVSGAGSHVG QTHMGSLFGA ASLSPGLNGY ELNGEPDRKT SSIAALRMKA KEHSAAISWA T
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ALX4 ALX homeobox 4 [ Homo sapiens ]
• Official Symbol: ALX4
• Synonyms: ALX4; ALX homeobox 4; aristaless like homeobox 4 , parietal foramina 2 , PFM2; homeobox protein aristaless-like 4; FPP; KIAA1788; PFM; aristaless-like homeobox 4; homeodomain transcription factor ALX4; FND2
• Gene ID: 60529
• mRNA Refseq: NM_021926
• Protein Refseq: NP_068745
• MIM: 605420
• UniProt ID: Q9H161

Reviews

01/03/2019

Compatibility with small sample volumes for limited samples.

03/05/2018

High specificity in protein target identification.

01/04/2017

Stable and reliable reagents for reproducible results.

Q&As

What are the protein-protein interactions of ALX4, and how do these interactions contribute to its function in regulating gene expression and cellular processes?

ALX4 interacts with various co-regulatory proteins and transcription factors, forming complexes that modulate gene expression and cellular processes critical for embryonic development.

What are the DNA binding motifs recognized by ALX4, and how do they contribute to its transcriptional regulatory activity?

ALX4 recognizes specific DNA binding motifs, such as homeobox motifs, and binds to regulatory regions of target genes to regulate their transcriptional activity.

What are the functional consequences of gain-of-function or loss-of-function mutations in ALX4, and how do they impact embryonic development and tissue morphogenesis?

Gain-of-function or loss-of-function mutations in ALX4 can disrupt normal embryonic development, leading to craniofacial abnormalities, limb defects, or skeletal malformations.

What are the epigenetic modifications associated with ALX4 binding sites, and how do they influence its transcriptional activity and downstream gene expression?

ALX4 binding sites are associated with specific epigenetic modifications, such as DNA methylation or histone modifications, which contribute to the regulation of ALX4-mediated transcriptional activity.

How does ALX4 interact with signaling pathways, such as BMP, Wnt, or FGF signaling, to modulate gene expression and cellular behaviors during embryonic development?

ALX4 integrates with signaling pathways, such as BMP, Wnt, or FGF signaling, to modulate gene expression and influence cellular behaviors during embryogenesis.

Can ALX4 be targeted pharmacologically or genetically to modulate its activity, and what are the potential therapeutic implications?

Pharmacological or genetic modulation of ALX4 activity may have therapeutic implications for craniofacial disorders, skeletal dysplasias, or regenerative medicine, but further studies are needed to explore these possibilities.

How does ALX4 interact with other transcription factors and co-regulators to form transcriptional complexes that control gene expression and cellular fate during embryonic development?

ALX4 forms transcriptional complexes with other transcription factors and co-regulators, such as MSX1 or RUNX2, to coordinate gene expression programs essential for craniofacial development, skeletal patterning, and limb morphogenesis.

What is the spatiotemporal expression pattern of ALX4 during embryonic development, and in which tissues and cell types is it predominantly expressed?

ALX4 exhibits a dynamic spatiotemporal expression pattern during embryonic development, with prominent expression in craniofacial structures, limb buds, and axial skeleton.

How does ALX4 regulate cell proliferation, differentiation, and migration during embryogenesis, and what are the underlying molecular mechanisms?

ALX4 regulates cell proliferation, differentiation, and migration during embryogenesis through various molecular mechanisms, including interactions with cell cycle regulators, morphogen gradients, or cytoskeletal components.

What are the downstream target genes regulated by ALX4, and how does it modulate their expression to influence cellular processes during development?

ALX4 regulates the expression of genes involved in craniofacial development, skeletal patterning, and limb formation, including those encoding transcription factors, extracellular matrix components, or signaling molecules.