Recombinant Full Length Human FLT3 Protein, C-Flag-tagged

• Cat.No.: FLT3-984HFL
• Product Overview: Recombinant Full Length Human FLT3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 112.7 kDa
• AA Sequence: MPALARGGGQLPLLVVFSAMIFGTI TNQDLPVIKCVLINHKNNDSSVGKS SSYPMVSESPEDLGCALRPQ SSGTVYEAAAVEVDVSASITLQVLV DAPGNISCLWVFKHSSLNCQPHFDL QNRGVVSMVILKMTETQAGE YLLFIQSEATNYTILFTVSIRNTLL YTLRRPYFRKMENQDALVCISESVP EPIVEWVLCDSQGESCKEES PAVVKKEEKVLHELFGMDIRCCARN ELGRECTRLFTIDLNQTPQTTLPQL FLKVGEPLWIRCKAVHVNHG FGLTWELENKALEEGNYFEMSTYST NRTMIRILFAFVSSVARNDTGYYTC SSSKHPSQSALVTIVEKGFI NATNSSEDYEIDQYEEFCFSVRFKA YPQIRCTWTFSRKSFPCEQKGLDNG YSISKFCNHKHQPGEYIFHA ENDDAQFTKMFTLNIRRKPQVLAEA SASQASCFSDGYPLPSWTWKKCSDK SPNCTEEITEGVWNRKANRK VFGQWVSSSTLNMSEAIKGFLVKCC AYNSLGTSCETILLNSPGPFPFIQD NISFYATIGVCLLFIVVLTL LICHKYKKQFRYESQLQMVQVTGSS DNEYFYVDFREYEYDLKWEFPRENL EFGKVLGSGAFGKVMNATAY GISKTGVSIQVAVKMLKEKADSSER EALMSELKMMTQLGSHENIVNLLGA CTLSGPIYLIFEYCCYGDLL NYLRSKREKFHRTWTEIFKEHNFSF YPTFQSHPNSSMPGSREVQIHPDSD QISGLHGNSFHSEDEIEYEN QKRLEEEEDLNVLTFEDLLCFAYQV AKGMEFLEFKSCVHRDLAARNVLVT HGKVVKICDFGLARDIMSDS NYVVRGNARLPVKWMAPESLFEGIY TIKSDVWSYGILLWEIFSLGVNPYP GIPVDANFYKLIQNGFKMDQ PFYATEEIYIIMQSCWAFDSRKRPS FPNLTSFLGCQLADAEEAMYQNVDG RVSECPHTYQNRRPFSREMD
  LGLLSPQAQVEDSTRTRPLEQKLIS EEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane
• Protein Pathways: Acute myeloid leukemia, Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Pathways in cancer

Gene Information

• Gene Name: FLT3 fms related receptor tyrosine kinase 3 [ Homo sapiens (human) ]
• Official Symbol: FLT3
• Synonyms: FLK2; STK1; CD135; FLK-2
• Gene ID: 2322
• mRNA Refseq: NM_004119.3
• Protein Refseq: NP_004110.2
• MIM: 136351
• UniProt ID: P36888