Recombinant Full Length Human PUM1 Protein, C-Flag-tagged

• Cat.No.: PUM1-930HFL
• Product Overview: Recombinant Full Length Human PUM1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 126.3 kDa
• AA Sequence: MSVACVLKRKAVLWQDSFSPHLKHH PQEPANPNMPVVLTSGTGSQAQPQP AANQALAAGTHSSPVPGSIG VAGRSQDDAMVDYFFQRQHGEQLGG GGSGGGGYNNSKHRWPTGDNIHAEH QVRSMDELNHDFQALALEGR AMGEQLLPGKKFWETDESSKDGPKG IFLGDQWRDSAWGTSDHSVSQPIMV QRRPGQSFHVNSEVNSVLSP RSESGGLGVSMVEYVLSSSPGDSCL RKGGFGPRDADSDENDKGEKKNKGT FDGDKLGDLKEEGDVMDKTN GLPVQNGIDADVKDFSRTPGNCQNS ANEVDLLGPNQNGSEGLAQLTSTNG AKPVEDFSNMESQSVPLDPM EHVGMEPLQFDYSGTQVPVDSAAAT VGLFDYNSQQQLFQRPNALAVQQLT AAQQQQYALAAAHQPHIGLA PAAFVPNPYIISAAPPGTDPYTAGL AAAATLGPAVVPHQYYGVTPWGVYP ASLFQQQAAAAAAATNSANQ QTTPQAQQGQQQVLRGGASQRPLTP NQNQQGQQTDPLVAAAAVNSALAFG QGLAAGMPGYPVLAPAAYYD QTGALVVNAGARNGLGAPVRLVAPA PVIISSSAAQAAVAAAAASANGAAG GLAGTTNGPFRPLGTQQPQP QPQQQPNNNLASSSFYGNNSLNSNS QSSSLFSQGSAQPANTSLGFGSSSS LGATLGSALGGFGTAVANSN TGSGSRRDSLTGSSDLYKRTSSSLT PIGHSFYNGLSFSSSPGPVGMPLPS QGPGHSQTPPPSLSSHGSSS SLNLGGLTNGSGRYISAAPGAEAKY RSASSASSLFSPSSTLFSSSRLRYG MSDVMPSGRSRLLEDFRNNR YPNLQLREIAGHIMEFSQDQHGSRF IQLKLERATPAERQLVFNEILQAAY QLMVDVFGNYVIQKFFEFGS LEQKLALAERIRGHVLSLALQMYGC RVIQKALEFIPSDQQNEMVRELDGH VLKCVKDQNGNHVVQKCIEC VQPQSLQFIIDAFKGQVFALSTHPY GCRVIQRILEHCLPDQTLPILEELH QHTEQLVQDQYGNYVIQHVL EHGRPEDKSKIVAEIRGNVLVLSQH KFASNVVEKCVTHASRTERAVLIDE VCTMNDGPHSALYTMMKDQY ANYVVQKMIDVAEPGQRKIVMHKIR PHIATLRKYTYGKHILAKLEKYYMK NGVDLGPICGPPNGIITRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: PUM1 pumilio RNA binding family member 1 [ Homo sapiens (human) ]
• Official Symbol: PUM1
• Synonyms: PUMH; HSPUM; PUMH1; PUML1; SCA47
• Gene ID: 9698
• mRNA Refseq: NM_014676.3
• Protein Refseq: NP_055491.1
• MIM: 607204
• UniProt ID: Q14671

Reviews

08/25/2023

PUM1 performed well in the experiment and met my expectations.

02/07/2022

PUM1 is reliable, reliable and reliable.

11/14/2021

It has the expected biological activity, such as enzyme activity, receptor binding activity, etc., and performs well.

Q&As

Is there a relationship between PUM1's structure and function?

PUM1's structure determines its ability to bind to RNA and its post-transcriptional regulatory role. The study of its structure can help us understand its function and mechanism of action.

How is PUM1 expression level associated with disease?

Recently published articles indicate that PUM1 expression levels are associated with the occurrence and development of certain cancers. High expression of PUM1 may promote tumor growth and spread.

What factors should be considered in drug development for PUM1?

Drug development for PUM1 requires consideration of its specificity, safety, pharmacokinetic properties, and potential side effects. In addition, issues such as the dosage form, route of administration and design of clinical trials need to be considered.

To illustrate the specific role of PUM1 in cancer？

In cancer, PUM1 may play a role by promoting processes such as the growth, migration and invasion of tumor cells. It could be a potential target for cancer treatment.

What are the interactions of PUM1 with other proteins?

It can interact with a variety of other proteins, including transcription factors and RNA-degrading enzymes. These interactions allow it to regulate the expression levels of specific genes.

What are the treatment strategies for PUM1?

Therapeutic strategies for PUM1 may include inhibiting its expression, blocking its binding to specific RNA sequences, or regulating its associated signal transduction pathways. These methods may have potential applications in cancer treatment.