Recombinant Mouse Pum1 Protein, Myc/DDK-tagged

• Cat.No.: Pum1-5257M
• Product Overview: Purified recombinant protein of mouse full-length pumilio RNA-binding family member 1 (Pum1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation. Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm. Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery. In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation. Binds specifically to miRNA MIR199A precursor, with PUM2, regulates miRNA MIR199A expression at a postranscriptional level.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 127 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pum1 pumilio RNA-binding family member 1 [ Mus musculus (house mouse) ]
• Official Symbol: Pum1
• Synonyms: PUM1; pumilio 1 (Drosophila); pumilio homolog 1; Pumm; AA517475; mKIAA0099
• Gene ID: 80912
• mRNA Refseq: NM_001159604
• Protein Refseq: NP_001153076
• UniProt ID: Q80U78

Reviews

08/25/2023

PUM1 performed well in the experiment and met my expectations.

02/07/2022

PUM1 is reliable, reliable and reliable.

11/14/2021

It has the expected biological activity, such as enzyme activity, receptor binding activity, etc., and performs well.

Q&As

Is there a relationship between PUM1's structure and function?

PUM1's structure determines its ability to bind to RNA and its post-transcriptional regulatory role. The study of its structure can help us understand its function and mechanism of action.

How is PUM1 expression level associated with disease?

Recently published articles indicate that PUM1 expression levels are associated with the occurrence and development of certain cancers. High expression of PUM1 may promote tumor growth and spread.

What factors should be considered in drug development for PUM1?

Drug development for PUM1 requires consideration of its specificity, safety, pharmacokinetic properties, and potential side effects. In addition, issues such as the dosage form, route of administration and design of clinical trials need to be considered.

To illustrate the specific role of PUM1 in cancer？

In cancer, PUM1 may play a role by promoting processes such as the growth, migration and invasion of tumor cells. It could be a potential target for cancer treatment.

What are the interactions of PUM1 with other proteins?

It can interact with a variety of other proteins, including transcription factors and RNA-degrading enzymes. These interactions allow it to regulate the expression levels of specific genes.

What are the treatment strategies for PUM1?

Therapeutic strategies for PUM1 may include inhibiting its expression, blocking its binding to specific RNA sequences, or regulating its associated signal transduction pathways. These methods may have potential applications in cancer treatment.