Recombinant Human PUM1, His-tagged

• Cat.No.: PUM1-31264TH
• Product Overview: Recombinant fragment, corresponding to amino acids 1005-1186 of Human Pumilio 1 with N terminal His tag; 182 amino acids, 25kDa.

Specification

• Description: This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3 untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:Reconstitute with 89 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Store at 4°C. Upon reconstitution store at -80oC.
• Sequences of amino acids: YGCRVIQRILEHCLPDQTLPILEEL HQHTEQLVQDQYGNY VIQHVLEHGRPEDKSKIVAEIRGNV LVLSQHKFASNVV EKCVTHASRTERAVLIDEVCTMNDG PHSALYTMMKDQYANYVVQKMIDVA EPGQRKIVMHKIRPHIATLRKYTYG KHI LAKLEKYYMKNGVDLGPICGPPNGI I

Gene Information

• Gene Name: PUM1 pumilio homolog 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: PUM1
• Synonyms: PUM1; pumilio homolog 1 (Drosophila); pumilio (Drosophila) homolog 1; pumilio homolog 1; KIAA0099; PUMH1
• Gene ID: 9698
• mRNA Refseq: NM_001020658
• Protein Refseq: NP_001018494
• MIM: 607204
• Uniprot ID: Q14671
• Chromosome Location: 1p35.2
• Pathway: Clathrin derived vesicle budding, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; Membrane Trafficking, organism-specific biosystem; trans-Golgi Network Vesicle Budding, organism-specific biosystem
• Function: RNA binding; binding

Reviews

08/25/2023

PUM1 performed well in the experiment and met my expectations.

02/07/2022

PUM1 is reliable, reliable and reliable.

11/14/2021

It has the expected biological activity, such as enzyme activity, receptor binding activity, etc., and performs well.

Q&As

Is there a relationship between PUM1's structure and function?

PUM1's structure determines its ability to bind to RNA and its post-transcriptional regulatory role. The study of its structure can help us understand its function and mechanism of action.

How is PUM1 expression level associated with disease?

Recently published articles indicate that PUM1 expression levels are associated with the occurrence and development of certain cancers. High expression of PUM1 may promote tumor growth and spread.

What factors should be considered in drug development for PUM1?

Drug development for PUM1 requires consideration of its specificity, safety, pharmacokinetic properties, and potential side effects. In addition, issues such as the dosage form, route of administration and design of clinical trials need to be considered.

To illustrate the specific role of PUM1 in cancer？

In cancer, PUM1 may play a role by promoting processes such as the growth, migration and invasion of tumor cells. It could be a potential target for cancer treatment.

What are the interactions of PUM1 with other proteins?

It can interact with a variety of other proteins, including transcription factors and RNA-degrading enzymes. These interactions allow it to regulate the expression levels of specific genes.

What are the treatment strategies for PUM1?

Therapeutic strategies for PUM1 may include inhibiting its expression, blocking its binding to specific RNA sequences, or regulating its associated signal transduction pathways. These methods may have potential applications in cancer treatment.