Recombinant Full Length Human RBX1 Protein, C-Flag-tagged

• Cat.No.: RBX1-1980HFL
• Product Overview: Recombinant Full Length Human RBX1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 12.1 kDa
• AA Sequence: MAAAMDVDTPSGTNSGAGKKRFEVK KWNAVALWAWDIVVDNCAICRNHIM DLCIECQANQASATSEECTV AWGVCNHAFHFHCISRWLKTRQVCP LDNREWEFQKYGH TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Nucleotide excision repair, Oocyte meiosis, Pathways in cancer, Renal cell carcinoma, TGF-beta signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathwa

Gene Information

• Gene Name: RBX1 ring-box 1 [ Homo sapiens (human) ]
• Official Symbol: RBX1
• Synonyms: ROC1; RNF75; BA554C12.1
• Gene ID: 9978
• mRNA Refseq: NM_014248.4
• Protein Refseq: NP_055063.1
• MIM: 603814
• UniProt ID: P62877

Reviews

03/01/2020

Efficiency and precision, vital for research quality.

11/02/2019

Critical for decision-making, accelerates research productivity.

04/04/2017

Enhances research credibility, a cornerstone of our research.

Q&As

How does RBX1 contribute to proteasome-mediated degradation?

It facilitates the attachment of ubiquitin, directing proteins to the proteasome for breakdown.

What potential therapeutic applications arise from targeting RBX1 in diseases?

Targeting RBX1 offers potential for treating diseases with dysregulated protein turnover, including cancer.

What is the impact of altered RBX1 activity on cancer development?

Altered RBX1 activity is associated with cancer progression, influencing cell proliferation and survival.

What role does RBX1 play in cell cycle regulation?

RBX1 plays a role in cell cycle control by regulating the degradation of key cycle-related proteins.

How do genetic variations in RBX1 affect cellular processes?

Genetic mutations in RBX1 can lead to disruptions in protein turnover, affecting various cellular functions.

What is the primary role of RBX1 in protein ubiquitination?

RBX1, a RING-finger protein, is crucial for tagging proteins with ubiquitin, marking them for degradation.

How does RBX1 interact with other components of the ubiquitin-proteasome system?

RBX1 works with other ubiquitin-proteasome system components, orchestrating protein degradation.