Recombinant Full Length Human SLC10A1 Protein, C-Flag-tagged

• Cat.No.: SLC10A1-413HFL
• Product Overview: Recombinant Full Length Human SLC10A1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the sodium/bile acid cotransporter family, which are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids; the ileal sodium/bile acid cotransporter with an apical cell localization that absorbs bile acids from the intestinal lumen, bile duct and kidney, and the liver-specific sodium/bile acid cotransporter, represented by this protein, that is found in the basolateral membranes of hepatocytes. Bile acids are the catabolic product of cholesterol metabolism, hence this protein is important for cholesterol homeostasis.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 37.9 kDa
• AA Sequence: MEAHNASAPFNFTLPPNFGKRPTDL ALSVILVFMLFFIMLSLGCTMEFSK IKAHLWKPKGLAIALVAQYG IMPLTAFVLGKVFRLKNIEALAILV CGCSPGGNLSNVFSLAMKGDMNLSI VMTTCSTFCALGMMPLLLYI YSRGIYDGDLKDKVPYKGIVISLVL VLIPCTIGIVLKSKRPQYMRYVIKG GMIIILLCSVAVTVLSAINV GKSIMFAMTPLLIATSSLMPFIGFL LGYVLSALFCLNGRCRRTVSMETGC QNVQLCSTILNVAFPPEVIG PLFFFPLLYMIFQLGEGLLLIAIFW CYEKFKTPKDKTKMIYTAATTEETI PGALGNGTYKGEDCSPCTASGPTRT RRLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane

Gene Information

• Gene Name: SLC10A1 solute carrier family 10 member 1 [ Homo sapiens (human) ]
• Official Symbol: SLC10A1
• Synonyms: NTCP; FHCA2
• Gene ID: 6554
• mRNA Refseq: NM_003049.4
• Protein Refseq: NP_003040.1
• MIM: 182396
• UniProt ID: Q14973