Recombinant Full Length Rat Long-Chain-Fatty-Acid--Coa Ligase 6(Acsl6) Protein, His-Tagged

• Cat.No.: RFL-24157RF
• Product Overview: Recombinant Full Length Rat Long-chain-fatty-acid--CoA ligase 6(Acsl6) Protein (P33124) (1-697aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Rattus norvegicus (Rat)
• Tag: His
• Form: Lyophilized powder
• Protein length: Full Length (1-697)
• AA Sequence: MQTQEILRILRLPELSDLGQFFRSL SATTLVSMGALAAILAYWLTHRPKA LQPPCNLLMQSEEVEDSGGARRSVI GDCTQLLTHYYDDARTMYQVFRRGL SISGNGPCLGFRKPEQPYQWLSYQE VAKRAEFLGSGLLQHDCKVGTEQFI GVFAQNRPEWIIAELACYTYSMVVV PLYDTLGPGSIRYIINTADICTVIV DKPHKAILLLEHVERKETPGLKLVI LMEPFDDALRERGKKCGVDIKSMQA IEDSGQENHRVPVPPRPDDLSIVCF TSGTTGNPKGAMLTHGNVVADFSGF LKVTESQWAPTCADVHFSYLPLAHM FERMVQSVVYCHGGRVGFFQGDIRL LSDDMKALRPTIFPVVPRLLNRMYD KIFHQADTSLKRWLLEFAAKRKQAE VRSGIIRNNSIWDELFFNKIQASLG GHVRMIVTGAAPASPTVLGFLRAAL GCQVYEGYGQTECTAGCTFTTPGDW TSGHVGAPLPCNHIKLVDAEELNYW TSKGEGEICVKGPNVFKGYLKDEDR TKEALDSDGWLHTGDIGKWLPEGTL KIIDRKKHIFKLAQGEYVAPEKIEN IYIRSEPVAQIYVHGDSLKAFLVGI VVPDPEVMPCWAQKKGIEGNYQELC KSKELKKAILDDMVMLGKESGLHSF EQVKAIYIHCDMFSVQNGLLTPTLK AKRPELREYFKKQIEELYSISM
• Purity: Greater than 90% as determined by SDS-PAGE.
• Applications: SDS-PAGE
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: Acsl6
• Synonyms: Acsl6; Acs2; Facl6; Long-chain-fatty-acid--CoA ligase 6; Arachidonate--CoA ligase; Long-chain acyl-CoA synthetase 6; LACS 6; Long-chain-fatty-acid--CoA ligase, brain isozyme
• UniProt ID: P33124

Gene Information

• Gene Name: Acsl6
• Synonyms: Acsl6; Acs2; Facl6; Long-chain-fatty-acid--CoA ligase 6; Arachidonate--CoA ligase; Long-chain acyl-CoA synthetase 6; LACS 6; Long-chain-fatty-acid--CoA ligase, brain isozyme
• UniProt ID: P33124

Reviews

03/27/2020

I'm thrilled with the performance of this recombinant protein. It surpassed my expectations in terms of purity and yield.

10/22/2019

I'm amazed at the performance of this recombinant protein. It worked flawlessly in my assays, and the results were reproducible.

06/22/2019

The high-quality protein allowed me to achieve robust and reliable results. I'm extremely satisfied with my purchase.

06/13/2018

Overall, I highly recommend this recombinant protein and the manufacturer for their excellent product and service.

05/04/2018

The recombinant protein was delivered promptly, and the packaging ensured its stability. The purity and activity were excellent, and the product performed consistently.

Q&As

How does ACSL6 expression change with aging?

ACSL6 expression has been shown to decrease with age, which may contribute to age-related metabolic dysfunction.

How does ACSL6 affect insulin sensitivity?

Studies have shown that increased ACSL6 expression in adipose tissue is associated with improved insulin sensitivity and decreased risk of developing type 2 diabetes.

What is the tissue distribution of ACSL6 expression?

ACSL6 is expressed in a variety of tissues, including adipose tissue, liver, muscle, and heart.

What is the role of ACSL6 in the brain?

ACSL6 is involved in the uptake and utilization of fatty acids in the brain, which plays a critical role in maintaining brain function.

How is the ACSL6 protein regulated at the transcriptional level?

ACSL6 gene expression is regulated by various transcription factors, including PPARs, SREBP-1c, and LXR.

How is ACSL6 expression regulated in response to changes in cellular energy status?

ACSL6 expression is regulated by AMPK, a key cellular energy sensor that activates ACSL6 transcription in response to low energy status.

What is the role of the C-terminal acyl-CoA synthetase domain of ACSL6?

The C-terminal domain of ACSL6 is responsible for the catalytic activity of the enzyme, which involves the activation of long-chain fatty acids by attaching them to coenzyme A.

What is the role of ACSL6 in lipid droplet formation?

ACSL6 is involved in the synthesis and storage of triacylglycerols in lipid droplets, which plays a critical role in maintaining lipid homeostasis.

How is ACSL6 involved in mitochondrial function?

ACSL6 is involved in the transport of fatty acids into the mitochondria, which is necessary for mitochondrial energy production.

How does the AMP-binding domain of ACSL6 regulate enzyme activity?

The AMP-binding domain of ACSL6 binds to AMP, which can activate the enzyme and increase its activity.