Recombinant Human ACAP2 293 Cell Lysate
Cat.No. : | ACAP2-9109HCL |
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Description : | Antigen standard for ArfGAP with coiled-coil, ankyrin repeat and PH domains 2 (ACAP2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ACAP2 ArfGAP with coiled-coil, ankyrin repeat and PH domains 2 [ Homo sapiens ] |
Official Symbol : | ACAP2 |
Synonyms : | ACAP2; ArfGAP with coiled-coil, ankyrin repeat and PH domains 2; centaurin, beta 2 , CENTB2; arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2; CNT B2; KIAA0041; centaurin-beta-2; centaurin, beta 2; Arf GAP with coiled coil, ANK repeat and PH domains 2; CENTB2; CNT-B2; |
Gene ID : | 23527 |
mRNA Refseq : | NM_012287 |
Protein Refseq : | NP_036419 |
MIM : | 607766 |
UniProt ID : | Q15057 |
Chromosome Location : | 3q29 |
Pathway : | Arf6 signaling events, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; |
Function : | ARF GTPase activator activity; metal ion binding; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
ACAP2-0003H | Recombinant Human ACAP2 Protein, GST-Tagged | +Inquiry |
ACAP2-97R | Recombinant Rat ACAP2 Protein, His (Fc)-Avi-tagged | +Inquiry |
ACAP2-29R | Recombinant Rhesus Macaque ACAP2 Protein, His (Fc)-Avi-tagged | +Inquiry |
ACAP2-243M | Recombinant Mouse ACAP2 Protein, His (Fc)-Avi-tagged | +Inquiry |
Acap2-1488M | Recombinant Mouse Acap2 Protein, Myc/DDK-tagged | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Recombinant ACAP2 protein can be used for various applications, such as biochemical assays, protein-protein interaction studies, and drug discovery. It can also be used as an immunogen for antibody production or as a standard in ELISA assays.
Recombinant ACAP2 protein can be used in both in vitro and in vivo assays, depending on your research needs. In vitro assays can include biochemical assays, protein-protein interaction studies, and enzymatic assays, while in vivo assays can include cell-based assays or animal models.
ACAP2 is involved in the regulation of cytokinesis by controlling the activity of RhoA, a small GTPase that plays a critical role in the formation of the contractile ring. ACAP2 interacts with RhoA and regulates its activity, which is necessary for the proper formation and contraction of the contractile ring.
ACAP2 is involved in the regulation of clathrin-mediated endocytosis (CME) by controlling the formation of clathrin-coated vesicles. It interacts with multiple proteins such as AP2, Eps15, and dynamin, and regulates the activity of Arf6 GTPase, which is essential for the formation of clathrin-coated vesicles.
Creative BioMart offers customization or modification services for recombinant ACAP2 protein, such as adding tags or modifying the protein sequence.
It depends on the specific product. Generally, the price is based on factors such as purity, quantity, and functional activity. Please submit an inquiry form for specific products, or contact our technical support staff for consultation.
The presence or absence of post-translational modifications in recombinant ACAP2 protein can vary depending on the expression system and the purification method used. We support custom production of recombinant proteins, and you can describe your specific research requirements when submitting the online inquiry form.
ACAP2 is involved in the regulation of cell migration by controlling the activity of Rac1, a small GTPase that plays a critical role in the formation of lamellipodia and membrane ruffling. ACAP2 interacts with Rac1 and regulates its activity, which is necessary for the formation of these structures during cell migration.
ACAP2 has been shown to play a critical role in the regulation of cell proliferation and survival, and its dysregulation has been linked to the development and progression of various types of cancer. ACAP2 has been shown to be upregulated in several types of cancer, including breast, lung, and liver cancer, and its inhibition has been proposed as a potential therapeutic strategy for cancer treatment.
Customer Reviews (3)
Write a reviewI am very satisfied with the recombinant human ACAP2 protein. The product was of high quality and helped me achieve excellent results.
I have had nothing but positive experiences with Creative BioMart. Their recombinant ACAP2 proteins are consistently high-quality and their customer service is excellent.
Thanks to Creative BioMart's recombinant ACAP2 proteins, my research has progressed much more quickly than I had anticipated.
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