Recombinant Human ACOT1 293 Cell Lysate

• Cat.No.: ACOT1-9091HCL

Specification

• Description: Antigen standard for acyl-CoA thioesterase 1 (ACOT1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ACOT1 acyl-CoA thioesterase 1 [ Homo sapiens ]
• Official Symbol: ACOT1
• Synonyms: ACOT1; acyl-CoA thioesterase 1; acyl-coenzyme A thioesterase 1; ACH2; CTE 1; LACH2; CTE-I; CTE-Ib; long chain acyl-CoA hydrolase; long chain acyl-CoA thioester hydrolase; inducible cytosolic acyl-coenzyme A thioester hydrolase; CTE-1
• Gene ID: 641371
• mRNA Refseq: NM_001037161
• Protein Refseq: NP_001032238
• MIM: 614313
• UniProt ID: Q86TX2
• Chromosome Location: 14q24.3
• Pathway: Biosynthesis of unsaturated fatty acids, organism-specific biosystem; Biosynthesis of unsaturated fatty acids, conserved biosystem; Fatty acid elongation, organism-specific biosystem; Fatty acid elongation, conserved biosystem; oleate biosynthesis II (animals), organism-specific biosystem; oleate biosynthesis II (animals), conserved biosystem; stearate biosynthesis I (animals), organism-specific biosystem
• Function: acyl-CoA hydrolase activity; carboxylesterase activity; hydrolase activity; palmitoyl-CoA hydrolase activity; thiolester hydrolase activity

Q&As

Are there any mutations in the ACOT1 gene associated with disease?

Several genetic mutations in the ACOT1 gene have been associated with metabolic disorders, including obesity and insulin resistance.

What is the subcellular localization of ACOT1 protein?

ACOT1 protein is localized in the cytoplasm of cells.

What is the role of ACOT1 in lipid metabolism?

ACOT1 plays a crucial role in lipid metabolism by hydrolyzing long-chain acyl-CoA thioesters, which are intermediates in the beta-oxidation of fatty acids, and releasing free fatty acids that can be utilized for energy production or other metabolic processes.

Is ACOT1 expressed in all tissues?

ACOT1 is expressed in a variety of tissues, including the liver, heart, skeletal muscle, and brain.

What is the physiological significance of ACOT1?

ACOT1 is important for energy metabolism and is involved in regulating lipid storage and utilization. It has also been implicated in the development of obesity, metabolic disorders and cancer.

Is ACOT1 a membrane-bound protein?

ACOT1 is a soluble protein that is not membrane-bound.