Recombinant Human ACOT1 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: ACOT1-323H
• Product Overview: ACOT1 MS Standard C13 and N15-labeled recombinant protein (NP_001032238) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating intracellular levels of acyl-CoAs, free fatty acids and CoASH. More active towards saturated and unsaturated long chain fatty acyl-CoAs (C12-C20).
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 46.1 kDa
• AA Sequence: MAATLILEPAGRCCWDEPVRIAVRG LAPEQPVTLRASLRDEKGALFQAHA RYRADTLGELDLERAPALGGSFAGL EPMGLLWALEPEKPLVRLVKRDVRT PLAVELEVLDGHDPDPGRLLCRVRH ERYFLPPGVRREPVRAGRVRGTLFL PPEPGPFPGIVDMFGTGGGLLEYRA SLLAGKGFAVMALAYYNYEDLPKTM ETLHLEYFEEAVNYLLSHPEVKGPG VGLLGISKGGELCLSMASFLKGITA AVVINGSVANVGGTLRYKGETLPPV GVNRNRIKVTKDGYADIVDVLNSPL EGPDQKSFIPVERAESTFLFLVGQD DHNWKSEFYANEACKRLQAHGRRKP QIICYPETGHYIEPPYFPLCRASLH ALVGSPIIWGGEPRAHAMAQVDAWK QLQTFFHKHLGGHEGTIPSKVTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ACOT1 acyl-CoA thioesterase 1 [ Homo sapiens (human) ]
• Official Symbol: ACOT1
• Synonyms: ACOT1; acyl-CoA thioesterase 1; acyl-coenzyme A thioesterase 1; ACH2; CTE 1; LACH2; CTE-I; CTE-Ib; long chain acyl-CoA hydrolase; long chain acyl-CoA thioester hydrolase; inducible cytosolic acyl-coenzyme A thioester hydrolase; CTE-1
• Gene ID: 641371
• mRNA Refseq: NM_001037161
• Protein Refseq: NP_001032238
• MIM: 614313
• UniProt ID: Q86TX2

Q&As

Are there any mutations in the ACOT1 gene associated with disease?

Several genetic mutations in the ACOT1 gene have been associated with metabolic disorders, including obesity and insulin resistance.

What is the subcellular localization of ACOT1 protein?

ACOT1 protein is localized in the cytoplasm of cells.

What is the role of ACOT1 in lipid metabolism?

ACOT1 plays a crucial role in lipid metabolism by hydrolyzing long-chain acyl-CoA thioesters, which are intermediates in the beta-oxidation of fatty acids, and releasing free fatty acids that can be utilized for energy production or other metabolic processes.

Is ACOT1 expressed in all tissues?

ACOT1 is expressed in a variety of tissues, including the liver, heart, skeletal muscle, and brain.

What is the physiological significance of ACOT1?

ACOT1 is important for energy metabolism and is involved in regulating lipid storage and utilization. It has also been implicated in the development of obesity, metabolic disorders and cancer.

Is ACOT1 a membrane-bound protein?

ACOT1 is a soluble protein that is not membrane-bound.