Recombinant Human ACP2 293 Cell Lysate

• Cat.No.: ACP2-9082HCL

Specification

• Description: Antigen standard for acid phosphatase 2, lysosomal (ACP2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ACP2 acid phosphatase 2, lysosomal [ Homo sapiens ]
• Official Symbol: ACP2
• Synonyms: ACP2; acid phosphatase 2, lysosomal; lysosomal acid phosphatase; LAP
• Gene ID: 53
• mRNA Refseq: NM_001131064
• Protein Refseq: NP_001124536
• MIM: 171650
• UniProt ID: P11117
• Chromosome Location: 11p11.2
• Pathway: Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Riboflavin metabolism, organism-specific biosystem; Riboflavin metabolism, conserved biosystem
• Function: acid phosphatase activity; hydrolase activity; phosphoprotein phosphatase activity; phosphotyrosine binding

Reviews

05/06/2021

Highly recommended!

10/02/2019

Great performance in ELISA.

05/02/2018

Excellent, we will definitely go back to buy this again!

04/23/2018

Fast delivery !

04/22/2018

Satisfied with five starts!

Q&As

What is the buffer solution of the ACP2?

100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

One study found the downregulation of ACP2 expression could significantly induce the HCT-116 and DLD1 cells to survive 5-FU treatment compared with control. What does this conlcusion imply?

The high expression of ACP2 in CRC patients was associated with increased sensitivity to 5-FU chemotherapy.

Could please simply descibe the post-translational modifications for ACP2 gene?

The membrane-bound form is converted to the soluble form by sequential proteolytic processing; First, the C-terminal cytoplasmic tail is removed; Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen.

What will happen if the enzyme inactive occur in this gene?

An enzymatically-inactive allele of this gene in mice showed severe growth retardation, hair-follicle abnormalities, and an ataxia-like phenotype.

What is the preformance in mice if ACP2 was deleted?

Mice lacking this gene showed multiple defects, including bone structure alterations, lysosomal storage defects, and an increased tendency towards seizures.

What you can discover if protein expression of lysosomal acid phosphatase 2 (ACP2) was increased in colorectal cancer among patients received chemotherapy?

Among patients who received chemotherapy, those with an high expression of ACP2 showed better survival in both stage II and III colorectal cancer than those with low ACP2 expression.

List 2 inferred drugs for ACP2 gene, and their status?

D-Phenylalanine - Approved, Experimental, Investigational. D-Tyrosine - Approved, Experimental, Investigational.

What is the formulation of the ACP2 protein solution?

ACP2 protein solution (1mg/ml) contains 20mM Tris-HCl (pH8.0) and 10% glycerol.

What is the stability of the ACP2?

Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

What are the top5 interacting proteins with ACP2 gene?

They are TRHR, GMDS, SGCA, FPGS, and BAZ1B