Recombinant Human ACP2 Protein, His-tagged

• Cat.No.: ACP2-46H
• Product Overview: Recombinant Human ACP2 Protein (Tyr40-Ala250) with N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene belongs to the histidine acid phosphatase family, which hydrolyze orthophosphoric monoesters to alcohol and phosphate. This protein is localized to the lysosomal membrane, and is chemically and genetically distinct from the red cell acid phosphatase. Mice lacking this gene showed multiple defects, including bone structure alterations, lysosomal storage defects, and an increased tendency towards seizures. An enzymatically-inactive allele of this gene in mice showed severe growth retardation, hair-follicle abnormalities, and an ataxia-like phenotype. Alternatively spliced transcript variants have been found for this gene. A C-terminally extended isoform is also predicted to be produced by the use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 28.5 kDa
  Accurate Molecular Mass: 29 kDa
• Protein length: Tyr40-Ala250
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: ACP2 acid phosphatase 2, lysosomal [ Homo sapiens (human) ]
• Official Symbol: ACP2
• Synonyms: ACP2; acid phosphatase 2, lysosomal; lysosomal acid phosphatase; LAP
• Gene ID: 53
• mRNA Refseq: NM_001131064
• Protein Refseq: NP_001124536
• MIM: 171650
• UniProt ID: P11117

Reviews

05/06/2021

Highly recommended!

10/02/2019

Great performance in ELISA.

05/02/2018

Excellent, we will definitely go back to buy this again!

04/23/2018

Fast delivery !

04/22/2018

Satisfied with five starts!

Q&As

What is the buffer solution of the ACP2?

100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

One study found the downregulation of ACP2 expression could significantly induce the HCT-116 and DLD1 cells to survive 5-FU treatment compared with control. What does this conlcusion imply?

The high expression of ACP2 in CRC patients was associated with increased sensitivity to 5-FU chemotherapy.

Could please simply descibe the post-translational modifications for ACP2 gene?

The membrane-bound form is converted to the soluble form by sequential proteolytic processing; First, the C-terminal cytoplasmic tail is removed; Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen.

What will happen if the enzyme inactive occur in this gene?

An enzymatically-inactive allele of this gene in mice showed severe growth retardation, hair-follicle abnormalities, and an ataxia-like phenotype.

What is the preformance in mice if ACP2 was deleted?

Mice lacking this gene showed multiple defects, including bone structure alterations, lysosomal storage defects, and an increased tendency towards seizures.

What you can discover if protein expression of lysosomal acid phosphatase 2 (ACP2) was increased in colorectal cancer among patients received chemotherapy?

Among patients who received chemotherapy, those with an high expression of ACP2 showed better survival in both stage II and III colorectal cancer than those with low ACP2 expression.

List 2 inferred drugs for ACP2 gene, and their status?

D-Phenylalanine - Approved, Experimental, Investigational. D-Tyrosine - Approved, Experimental, Investigational.

What is the formulation of the ACP2 protein solution?

ACP2 protein solution (1mg/ml) contains 20mM Tris-HCl (pH8.0) and 10% glycerol.

What is the stability of the ACP2?

Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

What are the top5 interacting proteins with ACP2 gene?

They are TRHR, GMDS, SGCA, FPGS, and BAZ1B