Recombinant Human ACSL6 Protein, GST-tagged

• Cat.No.: ACSL6-202H
• Product Overview: Human ACSL6 full-length ORF ( AAH47453, 1 a.a. - 622 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The protein encoded by this gene catalyzes the formation of acyl-CoA from fatty acids, ATP, and CoA, using magnesium as a cofactor. The encoded protein plays a major role in fatty acid metabolism in the brain. Translocations with the ETV6 gene are causes of myelodysplastic syndrome with basophilia, acute myelogenous leukemia with eosinophilia, and acute eosinophilic leukemia. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2011]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 94.16 kDa
• AA Sequence: MQTQEILRILRLPELGDLGQFFRSL SATTLDSGGARRSVIGSGPQLLTHY YDDARTMYQVFRRGLSISGNGPCLG FRKPKQPYQWLSYQEVADRAEFLGS GLLQHNCKACTDQFIGVFAQNRPEW IIVELACYTYSMVVVPLYDTLGPGA IRYIINTADISTVIVDKPQKAVLLL EHVERKETPGLKLIILMDPFEEALK ERGQKCGVVIKSMQAVEDCGQANHQ APVPPQPDDLSIVCFTSGTTGNPKG AMLTHGNVVADFSGFLKVTEGDIRL LSDDMKALCPTIFPVVPRLLNRMYD KIFSQANTPLKRWLLEFAAKRKQAE VRSGIIRNDSIWDELFFNKIQASLG GCVRMIVTGAAPASPTVLGFLRAAL GCQVYEGYGQTECTAGCTFTTPGDW TSGHVGAPLPCNHIKLVDVEELNYW ACKGEGEICVRGPNVFKGYLKDPDR TKEALDSDGWLHTGDIGKWLPAGTL KIIDRKKHIFKLAQGEYVAPEKIEN IYIRSQPVAQIYVHGDSLKAFLVGI VVPDPEVMPSWAQKRGIEGTYADLC TNKDLKKAILEDMVRLGKESGLHSF EQVKAIHIHSDMFSVQNGLLTPTLK AKRPELREYFKKQIEELYSISM
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ACSL6 acyl-CoA synthetase long-chain family member 6 [ Homo sapiens ]
• Official Symbol: ACSL6
• Synonyms: ACSL6; acyl-CoA synthetase long-chain family member 6; FACL6, fatty acid Coenzyme A ligase, long chain 6; long-chain-fatty-acid--CoA ligase 6; ACS2; KIAA0837; LACS2; LACS5; long fatty acyl-CoA synthetase 2; fatty-acid-Coenzyme A ligase, long-chain 6; FACL6; LACS 6; FLJ16173
• Gene ID: 23305
• mRNA Refseq: NM_001009185
• Protein Refseq: NP_001009185
• MIM: 604443
• UniProt ID: Q9UKU0

Reviews

03/27/2020

I'm thrilled with the performance of this recombinant protein. It surpassed my expectations in terms of purity and yield.

10/22/2019

I'm amazed at the performance of this recombinant protein. It worked flawlessly in my assays, and the results were reproducible.

06/22/2019

The high-quality protein allowed me to achieve robust and reliable results. I'm extremely satisfied with my purchase.

06/13/2018

Overall, I highly recommend this recombinant protein and the manufacturer for their excellent product and service.

05/04/2018

The recombinant protein was delivered promptly, and the packaging ensured its stability. The purity and activity were excellent, and the product performed consistently.

Q&As

How does ACSL6 expression change with aging?

ACSL6 expression has been shown to decrease with age, which may contribute to age-related metabolic dysfunction.

How does ACSL6 affect insulin sensitivity?

Studies have shown that increased ACSL6 expression in adipose tissue is associated with improved insulin sensitivity and decreased risk of developing type 2 diabetes.

What is the tissue distribution of ACSL6 expression?

ACSL6 is expressed in a variety of tissues, including adipose tissue, liver, muscle, and heart.

What is the role of ACSL6 in the brain?

ACSL6 is involved in the uptake and utilization of fatty acids in the brain, which plays a critical role in maintaining brain function.

How is the ACSL6 protein regulated at the transcriptional level?

ACSL6 gene expression is regulated by various transcription factors, including PPARs, SREBP-1c, and LXR.

How is ACSL6 expression regulated in response to changes in cellular energy status?

ACSL6 expression is regulated by AMPK, a key cellular energy sensor that activates ACSL6 transcription in response to low energy status.

What is the role of the C-terminal acyl-CoA synthetase domain of ACSL6?

The C-terminal domain of ACSL6 is responsible for the catalytic activity of the enzyme, which involves the activation of long-chain fatty acids by attaching them to coenzyme A.

What is the role of ACSL6 in lipid droplet formation?

ACSL6 is involved in the synthesis and storage of triacylglycerols in lipid droplets, which plays a critical role in maintaining lipid homeostasis.

How is ACSL6 involved in mitochondrial function?

ACSL6 is involved in the transport of fatty acids into the mitochondria, which is necessary for mitochondrial energy production.

How does the AMP-binding domain of ACSL6 regulate enzyme activity?

The AMP-binding domain of ACSL6 binds to AMP, which can activate the enzyme and increase its activity.