Recombinant Human ACSS2 293 Cell Lysate

• Cat.No.: ACSS2-9068HCL

Specification

• Description: Antigen standard for acyl-CoA synthetase short-chain family member 2 (ACSS2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ACSS2 acyl-CoA synthetase short-chain family member 2 [ Homo sapiens ]
• Official Symbol: ACSS2
• Synonyms: ACSS2; acyl-CoA synthetase short-chain family member 2; ACAS2, acetyl Coenzyme A synthetase 2 (ADP forming); acetyl-coenzyme A synthetase, cytoplasmic; AceCS; ACS; ACSA; dJ1161H23.1; acetate thiokinase; acetate-CoA ligase; acyl-activating enzyme; cytoplasmic acetyl-coenzyme A synthetase; acetyl-Coenzyme A synthetase 2 (ADP forming); ACAS2; ACECS; DKFZp762G026
• Gene ID: 55902
• mRNA Refseq: NM_001076552
• Protein Refseq: NP_001070020
• MIM: 605832
• UniProt ID: Q9NR19
• Chromosome Location: 20q11.22
• Pathway: Biological oxidations, organism-specific biosystem; Ethanol oxidation, organism-specific biosystem; Fatty Acid Beta Oxidation, organism-specific biosystem; Fatty Acid Biosynthesis, organism-specific biosystem; Glycolysis / Gluconeogenesis, organism-specific biosystem; Glycolysis / Gluconeogenesis, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: AMP binding; AMP binding; ATP binding; acetate-CoA ligase activity; acetate-CoA ligase activity; catalytic activity; ligase activity; nucleotide binding

Reviews

01/09/2022

It showed superior stability, retaining its activity even under challenging experimental conditions. It has significantly improved the reliability of our assays.

11/03/2021

It's a game-changer for any lab seeking efficiency.

09/17/2020

This protein is a rock star! It consistently delivers outstanding results with a smile.

06/12/2019

The quality-price ratio of this protein is truly commendable.

04/28/2018

Its reliable and reproducible results have facilitated precise data acquisition, supporting the validity and integrity of our experimental findings.

Q&As

What is the role of ACSS2 protein in liver metabolism?

ACSS2 protein is required for normal liver metabolism and the synthesis of ketone bodies, which are important energy sources during fasting and starvation

What is the effect of ACSS2 protein inhibition on the response to hypoxia?

Inhibition of ACSS2 protein activity sensitizes cancer cells to hypoxia, a condition of low oxygen availability that promotes tumor progression and resistance to therapy.

What is the relationship between ACSS2 protein and fatty acid metabolism?

ACSS2 protein plays a critical role in linking fatty acid metabolism to oxidative metabolism in the mitochondria of mammalian cells.

What is the role of ACSS2 protein in adipose tissue?

ACSS2 protein plays a key role in adipose tissue metabolism by regulating the production of adipokines, hormones that are involved in the regulation of energy homeostasis.

What is the relationship between ACSS2 protein and aging?

ACSS2 protein expression has been shown to decline with age, and the loss of ACSS2 protein activity may contribute to age-related metabolic dysfunction.

What is the relationship between ACSS2 protein and circadian rhythms?

ACSS2 protein expression is regulated by circadian rhythms, and ACSS2 protein activity may play a role in the circadian regulation of metabolism.

What is the relationship between ACSS2 protein and the gut-brain axis?

Emerging evidence suggests that ACSS2 protein may play a role in the gut-brain axis, which is the bidirectional communication between the gut microbiome and the central nervous system.

What is the relationship between ACSS2 protein and the gut microbiome?

ACSS2 protein expression is regulated by the gut microbiome, and ACSS2 protein activity may play a role in the interaction between the host and the microbiome.

What is the effect of ACSS2 protein deficiency on macrophage function?

Deficiency of ACSS2 protein activity in macrophages has been shown to impair phagocytosis and reduce the production of inflammatory cytokines.

What is the role of ACSS2 protein in the regulation of fatty acid oxidation?

ACSS2 protein is involved in the regulation of fatty acid oxidation by providing the acetyl-CoA needed for entry into the TCA cycle.