Recombinant Mouse Acss2 Protein, His-tagged

• Cat.No.: Acss2-68M
• Product Overview: Recombinant Mouse Acss2 Protein (Pro492-Gln701) with N-His tag was expressed in E. coli.

Specification

• Description: Enables acetate-CoA ligase activity and propionate-CoA ligase activity. Acts upstream of or within acetyl-CoA biosynthetic process. Located in cytoplasm. Is expressed in several structures, including alimentary system; genitourinary system; liver; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in orofacial cleft. Orthologous to human ACSS2 (acyl-CoA synthetase short chain family member 2).
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 27.1 kDa
  Accurate Molecular Mass: 31 kDa
• Protein length: Pro492-Gln701
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Acss2 acyl-CoA synthetase short-chain family member 2 [ Mus musculus (house mouse) ]
• Official Symbol: Acss2
• Synonyms: ACSS2; acyl-CoA synthetase short-chain family member 2; acetyl-coenzyme A synthetase, cytoplasmic; ACS; aceCS; acetate--CoA ligase; acyl-activating enzyme; acetyl-CoA synthetase 1; acetyl-Coenzyme A synthetase 1 (AMP forming); acetyl-Coenzyme A synthetase 2 (ADP forming); acetyl-Coenzyme A synthetase 2 (AMP forming); ACAS; Acs1; Acas1; Acas2; AceCS1; 1110017C11Rik
• Gene ID: 60525
• mRNA Refseq: NM_019811
• Protein Refseq: NP_062785
• UniProt ID: Q9QXG4

Reviews

01/09/2022

It showed superior stability, retaining its activity even under challenging experimental conditions. It has significantly improved the reliability of our assays.

11/03/2021

It's a game-changer for any lab seeking efficiency.

09/17/2020

This protein is a rock star! It consistently delivers outstanding results with a smile.

06/12/2019

The quality-price ratio of this protein is truly commendable.

04/28/2018

Its reliable and reproducible results have facilitated precise data acquisition, supporting the validity and integrity of our experimental findings.

Q&As

What is the role of ACSS2 protein in liver metabolism?

ACSS2 protein is required for normal liver metabolism and the synthesis of ketone bodies, which are important energy sources during fasting and starvation

What is the effect of ACSS2 protein inhibition on the response to hypoxia?

Inhibition of ACSS2 protein activity sensitizes cancer cells to hypoxia, a condition of low oxygen availability that promotes tumor progression and resistance to therapy.

What is the relationship between ACSS2 protein and fatty acid metabolism?

ACSS2 protein plays a critical role in linking fatty acid metabolism to oxidative metabolism in the mitochondria of mammalian cells.

What is the role of ACSS2 protein in adipose tissue?

ACSS2 protein plays a key role in adipose tissue metabolism by regulating the production of adipokines, hormones that are involved in the regulation of energy homeostasis.

What is the relationship between ACSS2 protein and aging?

ACSS2 protein expression has been shown to decline with age, and the loss of ACSS2 protein activity may contribute to age-related metabolic dysfunction.

What is the relationship between ACSS2 protein and circadian rhythms?

ACSS2 protein expression is regulated by circadian rhythms, and ACSS2 protein activity may play a role in the circadian regulation of metabolism.

What is the relationship between ACSS2 protein and the gut-brain axis?

Emerging evidence suggests that ACSS2 protein may play a role in the gut-brain axis, which is the bidirectional communication between the gut microbiome and the central nervous system.

What is the relationship between ACSS2 protein and the gut microbiome?

ACSS2 protein expression is regulated by the gut microbiome, and ACSS2 protein activity may play a role in the interaction between the host and the microbiome.

What is the effect of ACSS2 protein deficiency on macrophage function?

Deficiency of ACSS2 protein activity in macrophages has been shown to impair phagocytosis and reduce the production of inflammatory cytokines.

What is the role of ACSS2 protein in the regulation of fatty acid oxidation?

ACSS2 protein is involved in the regulation of fatty acid oxidation by providing the acetyl-CoA needed for entry into the TCA cycle.