Recombinant Human ADAMTS10 293 Cell Lysate

• Cat.No.: ADAMTS10-9032HCL

Specification

• Description: Antigen standard for ADAM metallopeptidase with thrombospondin type 1 motif, 10 (ADAMTS10) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADAMTS10 ADAM metallopeptidase with thrombospondin type 1 motif, 10 [ Homo sapiens ]
• Official Symbol: ADAMTS10
• Synonyms: ADAMTS10; ADAM metallopeptidase with thrombospondin type 1 motif, 10; a disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 10; A disintegrin and metalloproteinase with thrombospondin motifs 10; ADAM TS10; ADAMTS-10; ADAM-TS 10; zinc metalloendopeptidase; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 10; WMS; WMS1; ADAM-TS10
• Gene ID: 81794
• mRNA Refseq: NM_030957
• Protein Refseq: NP_112219
• MIM: 608990
• UniProt ID: Q9H324
• Chromosome Location: 19p13.3
• Function: metal ion binding; metalloendopeptidase activity; molecular_function; peptidase activity; zinc ion binding

Reviews

03/04/2021

Prompt dispatch and smooth delivery helped me to obtain satisfactory experimental results within a short period of time

06/30/2020

The ADAMTS10 protein purchased was suitable for WB experiments and the experiments went very well and satisfactory results were obtained

05/08/2019

The delivery is very fast, the protein activity is good, and it can show good experimental results.

Q&As

What are the applications of ADAMTS10 in the study of diseases?

ADAMTS10 is mainly involved in the study ofpancreatic cancer, breast cancer and lung cancer.

Does ADAMTS10 interact with fibrillin-1?

Yes, they interact with each other and are co-located in human skin.

Can ADAMTS10-1308M specify the product form?

Yes, we can ship according to the product form you specify.

Where is ADAMTS10 mainly expressed?

ADAMTS10 is mainly expressed in the lung, kidney and bone.

Does this promotion apply to all protein products and custom needs?

Yes, this promotion applies to all protein products and custom needs.

Is human ADAMTS10 protein in stock?

Human ADAMTS10 protein is in stock.

What proteins does ADAM10 cleave?

ADAM10 cleaves transmembrane proteins such as amyloid precursor protein, E-calmodulin, N-calmodulin and NOTCH.

Can the tag of Zebrafish ADAMTS10 be other than His?

We can customize the tag according to your requirements.

What happens when ADAM10 is highly expressed?

High expression of ADAM10 leads to Notch signal pathway activation and stimulation of VSMCs proliferation.

Do you offer other expression systems for ADAMTS10 protein expression?

We provide several different protein expression systems, including baculovirus-insect cell expression systems, cell-free in vitro protein expression systems, yeast expression systems, etc.