Recombinant Human ADAMTS8 293 Cell Lysate

• Cat.No.: ADAMTS8-9027HCL

Specification

• Description: Antigen standard for ADAM metallopeptidase with thrombospondin type 1 motif, 8 (ADAMTS8) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADAMTS8 ADAM metallopeptidase with thrombospondin type 1 motif, 8 [ Homo sapiens ]
• Official Symbol: ADAMTS8
• Synonyms: ADAMTS8; ADAM metallopeptidase with thrombospondin type 1 motif, 8; a disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 8; A disintegrin and metalloproteinase with thrombospondin motifs 8; ADAM TS8; FLJ41712; METH2; METH-2; METH-8; ADAMTS-8; ADAM-TS 8; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 8; ADAM-TS8
• Gene ID: 11095
• mRNA Refseq: NM_007037
• Protein Refseq: NP_008968
• MIM: 605175
• UniProt ID: Q9UP79
• Chromosome Location: 11q25
• Function: heparin binding; integrin binding; low affinity phosphate transmembrane transporter activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding

Reviews

06/02/2022

The technician modified my experimental protocol so that my experiment went smoothly.

06/15/2021

Very good after-sales service to get my experiments going!

01/05/2021

The protein composition was stable and concentrated, allowing me to obtain satisfactory experimental results.

Q&As

What is the biological function of ADAMTS8?

ADAMTS8 shows anti-angiogenesis and inhibition of endothelial cell production

What diseases is ADAMTS8 used to study?

ADAMTS8 is used in more studies of heart and cardiovascular diseases

What is ADAMTS8 also called?

ADAMTS8 used to be called METH-2.

Where is ADAMTS8 mainly expressed?

ADAMTS8 is predominantly expressed in the lungs and heart

What is the structure domain of ADAMTS8?

Pre-structural domains, metalloproteinase structural domains, disintegrin-like structural domains, central thrombospondin-like motifs, cysteine-rich structural domains, spacer region structural domains and C-terminal motifs