Recombinant Human Adenylate Kinase 3-like 1, His-tagged

• Cat.No.: AK3L1-102H
• Product Overview: Recombinant human AK3, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (259 aa, 29.3 kDa).

Specification

• Cat. No.: AK3L1-102H
• Description: Adenylate kinase (AK; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2.7.4.3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Five isozymes of adenylate kinase have been identified in vertebrates. AK1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while AK2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. AK3, called GTP: AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell.
• Source: E. coli.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM DTT, 20% glycerol.
• Molecular Weight: 29.3 kDa (259 aa).
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASK LLRAVILGPP GSGKGTVSQR IAQNFGLQHL SSGHFLRENI KASTEVGEMA KQYIEKSLLV PDHVITRLMM SELENRRGQH WLLDGFPRTL GQAEALDKIC EVDLVISLNI PFETLKDRLS RRWIHPPSGR VYNLDFNPPH VHGIDDVTGE PLVQQEDDKP EAVAARLRQY KDVAKPVIEL YKSRGVLHQF SGTETNKIWP YVYTLFSNKI TPIQSKEAY
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: AK3L1
• Synonyms: AK3L1; adenylate kinase 3-like 1; AK3L1 adenylate kinase 3-like 1; AK3; AK4; MGC166959; EC 2.7.4.3; OTTHUMP00000010594; ATP-AMP transphosphorylase; GTP:AMP phosphotransferase; Adenylate kinase 3-like; adenylate kinase 3; mitochondrial adenylate kinase-3; nucleoside-triphosphate-adenylate kinase.
• Gene ID: 205
• mRNA Refseq: NM_001005353.1
• Protein Refseq: NP_001005353.1
• MIM: 103030
• UniProt ID: P27144
• Chromosome Location: 1p31.3
• Pathway: Purine metabolism
• Function: ATP binding; GTP binding; adenylate kinase activity; nucleotide binding; transferase activity

Reviews

12/19/2017

Armed with the AK3L1 protein and the steadfast backing of the manufacturer's technical support, I am empowered to surmount any challenges that may impede my progress.

07/16/2017

With their profound expertise and prompt assistance, I am confident that any obstacles I encounter will be swiftly overcome.

02/25/2017

This invaluable support propels me towards scientific advancements within my field, contributing to the collective body of knowledge.

Q&As

How is AK3L1 protein produced in cells?

AK3L1 protein is produced through a process called translation, where the AK3L1 gene is transcribed into messenger RNA (mRNA) molecules. These mRNA molecules are then translated by ribosomes into the corresponding AK3L1 protein.

Can AK3L1 protein be used as a biomarker?

Currently, there is limited information on the use of AK3L1 protein as a biomarker. However, studies have suggested that AK3L1 expression levels could potentially be associated with certain diseases, such as cancer, and may have diagnostic or prognostic value.

Is AK3L1 protein well-studied?

Despite its potential importance in cellular energy metabolism, AK3L1 is relatively less studied compared to other proteins. Further research is needed to fully understand its functions and mechanisms of action.

Where is AK3L1 protein found?

AK3L1 protein is found in various tissues and organs, including the brain, heart, liver, and skeletal muscles.

Are there any known interacting proteins or pathways associated with AK3L1?

AK3L1 has been reported to interact with other proteins involved in energy metabolism pathways, such as AMPK and mitochondrial proteins. These interactions suggest the involvement of AK3L1 in cellular energy regulation.

How is AK3L1 protein regulated in cells?

The regulation of AK3L1 protein is not well understood. It is believed that AK3L1 expression and activity can be influenced by various factors, including cellular energy status and signaling pathways involved in metabolism.

Can AK3L1 protein be a target for drug development?

AK3L1 has been proposed as a potential target for drug development. Inhibition of AK3L1 activity has shown promise as a therapeutic strategy for certain types of cancer.

Are there any known inhibitors or activators of AK3L1 protein?

There are currently no specific inhibitors or activators of AK3L1 that have been developed for therapeutic use. However, studies have identified certain compounds that can modulate AK3L1 expression or activity, and further research is ongoing to explore their potential applications.

Are there any known interactions or binding partners of AK3L1 protein?

AK3L1 protein has been reported to interact with several other proteins involved in mitochondrial metabolism and signaling pathways. For example, it has been shown to bind to voltage-dependent anion channels (VDACs) and hexokinase, which are both involved in regulating mitochondrial function and energy metabolism.

Is AK3L1 protein localized in specific cellular compartments?

AK3L1 protein is primarily localized in the mitochondrial matrix, which is the central compartment of mitochondria. Its presence in this compartment suggests a role in cellular energy metabolism and ATP production.

Are there any known genetic variations or mutations in AK3L1 protein?

Yes, there are genetic variations and mutations observed in the AK3L1 gene. These variations could potentially affect the function or regulation of the protein, but more research is necessary to determine their significance.

Are there any known genetic diseases associated with AK3L1 protein mutations?

Currently, there are no known genetic diseases specifically associated with AK3L1 protein mutations. However, variations in the AK3L1 gene have been identified in genome-wide association studies (GWAS), indicating potential associations with certain diseases or traits, such as cancer susceptibility and metabolic disorders.

Is AK3L1 protein associated with any diseases?

Research suggests that AK3L1 may be involved in certain diseases, such as cancer and neurodegenerative disorders, although more studies are needed to fully understand its role in these conditions.

Is AK3L1 protein conserved across different species?

AK3L1 protein is highly conserved across different species, indicating its importance and functional significance. It shows high similarity in amino acid sequence and structural features among vertebrate species.