Recombinant Human AGL 293 Cell Lysate

• Cat.No.: AGL-8980HCL

Specification

• Description: Antigen standard for amylo-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 4 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AGL amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase [ Homo sapiens ]
• Official Symbol: AGL
• Synonyms: AGL; amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase; amylo 1, 6 glucosidase, 4 alpha glucanotransferase; glycogen debranching enzyme; glycogen storage disease type III; glycogen debrancher; amylo-1, 6-glucosidase, 4-alpha-glucanotransferase; GDE
• Gene ID: 178
• mRNA Refseq: NM_000028
• Protein Refseq: NP_000019
• MIM: 610860
• UniProt ID: P35573
• Chromosome Location: 1p21
• Pathway: Glucose metabolism, organism-specific biosystem; Glycogen Metabolism, organism-specific biosystem; Glycogen breakdown (glycogenolysis), organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of carbohydrates, organism-specific biosystem; Starch and sucrose metabolism, organism-specific biosystem
• Function: 4-alpha-glucanotransferase activity; amylo-alpha-1,6-glucosidase activity; cation binding; glycogen debranching enzyme activity; hydrolase activity, acting on glycosyl bonds; polysaccharide binding; polyubiquitin binding; protein binding; sugar binding; transferase activity, transferring glycosyl groups

Reviews

05/17/2019

 Notable improvements in cell proliferation were attributed to the protein product’s efficacy.

11/30/2018

 We observed a significant enhancement in enzyme activity using the protein product.

09/11/2018

 The protein product’s compatibility with various assays broadened the scope of our investigations.

08/25/2017

 Reliable protein-protein interaction studies were enabled by the protein product’s pure composition.

08/20/2017

 Minimal background noise in our assays indicated the protein product’s quality.

Q&As

Can AGL mutations lead to hypoglycemia?

Yes, AGL mutations can cause low blood sugar levels due to impaired glucose release.

Can AGL mutations lead to cardiomyopathy?

AGL mutations might contribute to cardiomyopathy due to glycogen accumulation.

Can AGL mutations cause muscle weakness?

Yes, AGL mutations can lead to muscle weakness due to impaired glycogen breakdown.

Is AGL's role conserved across species?

Research explores whether AGL's function is conserved in different organisms.

How does AGL influence cellular glycogen stores?

AGL activity ensures proper glycogen breakdown, maintaining cellular glycogen levels.

Is AGL research ongoing?

Yes, ongoing research uncovers AGL's roles in metabolism and related diseases.

How does AGL contribute to glycogen structure?

AGL's enzymatic activity alters glycogen structure by removing branches.

Can AGL be a target for therapeutic interventions?

AGL's role in glycogen metabolism makes it a potential therapeutic target.

How does AGL influence glycogen synthesis?

AGL's enzymatic role is in breaking down glycogen, not synthesizing it.

How does AGL dysfunction impact different tissues?

AGL dysfunction can affect various tissues due to glycogen storage disruptions.

How does AGL deficiency impact muscle cells?

AGL deficiency affects muscle cells due to impaired glycogen breakdown.

What's the significance of AGL research?

Research on AGL provides insights into glycogen metabolism and related diseases.

Is AGL activity regulated by hormones?

Yes, hormones regulate AGL activity to maintain glucose levels.

Is AGL involved in heart health?

Yes, AGL's role in glycogen breakdown can impact heart function and health.

How does AGL contribute to metabolic homeostasis?

AGL's role in glycogen breakdown helps maintain glucose homeostasis.

Is AGL deficiency inherited?

Yes, AGL deficiency is usually inherited in an autosomal recessive manner.

How does AGL deficiency impact energy availability?

AGL deficiency hinders glucose release from glycogen, affecting energy supply.

Can AGL mutations affect exercise tolerance?

Yes, AGL mutations can impact exercise tolerance due to limited energy supply.

Can AGL mutations lead to multi-organ involvement?

Yes, AGL mutations can impact multiple organs due to glycogen storage issues.

Can AGL mutations affect brain function?

AGL mutations can impact brain health due to glycogen storage issues in certain tissues.