Recombinant Mouse Agl Protein, His-tagged

• Cat.No.: Agl-93M
• Product Overview: Recombinant Mouse Agl Protein (Asp1197-Leu1532) with N-His tag was expressed in E. coli.

Specification

• Description: Enables glycogen debranching enzyme activity; polysaccharide binding activity; and polyubiquitin modification-dependent protein binding activity. Acts upstream of or within glycogen metabolic process. Located in cytoplasm; inclusion body; and nucleus. Is expressed in several structures, including alimentary system; central nervous system; heart; musculature; and sensory organ. Used to study glycogen storage disease III. Human ortholog(s) of this gene implicated in glycogen storage disease III. Orthologous to human AGL (amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase).
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 42.4 kDa
  Accurate Molecular Mass: 44 kDa
• Protein length: Asp1197-Leu1532
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Agl amylo-1,6-glucosidase, 4-alpha-glucanotransferase [ Mus musculus (house mouse) ]
• Official Symbol: Agl
• Synonyms: AGL; amylo-1,6-glucosidase, 4-alpha-glucanotransferase; glycogen debranching enzyme; C77197; AI850929; 1110061O17Rik; 9430004C13Rik; 9630046L06Rik
• Gene ID: 77559
• mRNA Refseq: NM_001081326
• Protein Refseq: NP_001074795
• UniProt ID: F8VPN4

Reviews

05/17/2019

 Notable improvements in cell proliferation were attributed to the protein product’s efficacy.

11/30/2018

 We observed a significant enhancement in enzyme activity using the protein product.

09/11/2018

 The protein product’s compatibility with various assays broadened the scope of our investigations.

08/25/2017

 Reliable protein-protein interaction studies were enabled by the protein product’s pure composition.

08/20/2017

 Minimal background noise in our assays indicated the protein product’s quality.

Q&As

Can AGL mutations lead to hypoglycemia?

Yes, AGL mutations can cause low blood sugar levels due to impaired glucose release.

Can AGL mutations lead to cardiomyopathy?

AGL mutations might contribute to cardiomyopathy due to glycogen accumulation.

Can AGL mutations cause muscle weakness?

Yes, AGL mutations can lead to muscle weakness due to impaired glycogen breakdown.

Is AGL's role conserved across species?

Research explores whether AGL's function is conserved in different organisms.

How does AGL influence cellular glycogen stores?

AGL activity ensures proper glycogen breakdown, maintaining cellular glycogen levels.

Is AGL research ongoing?

Yes, ongoing research uncovers AGL's roles in metabolism and related diseases.

How does AGL contribute to glycogen structure?

AGL's enzymatic activity alters glycogen structure by removing branches.

Can AGL be a target for therapeutic interventions?

AGL's role in glycogen metabolism makes it a potential therapeutic target.

How does AGL influence glycogen synthesis?

AGL's enzymatic role is in breaking down glycogen, not synthesizing it.

How does AGL dysfunction impact different tissues?

AGL dysfunction can affect various tissues due to glycogen storage disruptions.

How does AGL deficiency impact muscle cells?

AGL deficiency affects muscle cells due to impaired glycogen breakdown.

What's the significance of AGL research?

Research on AGL provides insights into glycogen metabolism and related diseases.

Is AGL activity regulated by hormones?

Yes, hormones regulate AGL activity to maintain glucose levels.

Is AGL involved in heart health?

Yes, AGL's role in glycogen breakdown can impact heart function and health.

How does AGL contribute to metabolic homeostasis?

AGL's role in glycogen breakdown helps maintain glucose homeostasis.

Is AGL deficiency inherited?

Yes, AGL deficiency is usually inherited in an autosomal recessive manner.

How does AGL deficiency impact energy availability?

AGL deficiency hinders glucose release from glycogen, affecting energy supply.

Can AGL mutations affect exercise tolerance?

Yes, AGL mutations can impact exercise tolerance due to limited energy supply.

Can AGL mutations lead to multi-organ involvement?

Yes, AGL mutations can impact multiple organs due to glycogen storage issues.

Can AGL mutations affect brain function?

AGL mutations can impact brain health due to glycogen storage issues in certain tissues.