Recombinant Human AKAP5 293 Cell Lysate
Cat.No. : | AKAP5-8939HCL |
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Description : | Antigen standard for A kinase (PRKA) anchor protein 5 (AKAP5) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | AKAP5 A kinase (PRKA) anchor protein 5 [ Homo sapiens ] |
Official Symbol : | AKAP5 |
Synonyms : | AKAP5; A kinase (PRKA) anchor protein 5; A-kinase anchor protein 5; AKAP75; AKAP79; AKAP-5; AKAP 79; A-kinase anchor protein 79 kDa; A-kinase anchor protein, 79kDa; A-kinase anchoring protein 75/79; cAMP-dependent protein kinase regulatory subunit II high affinity binding protein; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein; H21; |
Gene ID : | 9495 |
mRNA Refseq : | NM_004857 |
Protein Refseq : | NP_004848 |
MIM : | 604688 |
UniProt ID : | P24588 |
Chromosome Location : | 14q21-q24 |
Pathway : | Calcium signaling in the CD4+ TCR pathway, organism-specific biosystem; G Protein Signaling Pathways, organism-specific biosystem; Glutamate Binding, Activation of AMPA Receptors and Synaptic Plasticity, organism-specific biosystem; Integration of energy metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Neuronal System, organism-specific biosystem; Neurotransmitter Receptor Binding And Downstream Transmission In ThePostsynaptic Cell, organism-specific biosystem; |
Function : | adenylate cyclase binding; calmodulin binding; protein binding; protein kinase A binding; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (17)
Ask a questionAt present, AKAP5 is not established as a specific diagnostic marker for any diseases. However, ongoing research may uncover its potential diagnostic utility in certain conditions where AKAP5 dysregulation or altered expression has been observed.
AKAP5 contains multiple structural domains that contribute to its function. It has a long N-terminal region rich in potential protein-protein interaction sites, including amphipathic helices and an isoleucine-arginine motif. It also has a central domain that binds to the regulatory subunits of PKA and a C-terminal domain responsible for anchoring AKAP5 to various cellular structures.
Apart from PKA, AKAP5 interacts with multiple other molecules involved in signaling pathways. It can bind and recruit various enzymes, ion channels, receptors, and cytoskeletal proteins to specific subcellular locations. Some notable interacting partners of AKAP5 include phosphodiesterases, protein phosphatase 2B (calcineurin), NMDA receptors, L-type calcium channels, and adenylyl cyclases.
Yes, AKAP5 can interact with various isoforms of PKA by binding to their regulatory subunits. This allows for selective targeting of different PKA holoenzymes to specific subcellular locations.
Currently, there are no specific drugs or compounds that target AKAP5 directly. However, researchers are actively investigating the development of small molecules or peptides that can selectively disrupt AKAP5 interactions or modulate its functions.
AKAP5 plays a role in calcium signaling by anchoring PKA and calcium-related enzymes or channels. It is involved in regulating calcium-induced release of neurotransmitters and modulating NMDA receptor-mediated calcium influx.
Yes, AKAP5 knockout animal models have been generated to study its functions. These models have provided valuable insights into the role of AKAP5 in synaptic transmission, synaptic plasticity, and behavior.
Yes, AKAP5 can be phosphorylated by various kinases, including PKA itself. Phosphorylation of AKAP5 can regulate its localization, protein-protein interactions, and activity.
Some studies have identified genetic variations in AKAP5 that are associated with increased susceptibility to certain psychiatric disorders, including schizophrenia and bipolar disorder. However, more research is needed to fully understand the impact of these genetic variations on disease development and progression.
AKAP5 binds to the regulatory subunits of PKA, bringing the enzyme into close proximity with its substrate molecules. This interaction allows PKA to phosphorylate specific target proteins and regulate their function within the localized signaling microdomain.
Some studies highlight the involvement of AKAP5 in specific diseases and disorders. For instance, alterations in AKAP5 localization and function have been implicated in schizophrenia, bipolar disorder, and alcohol addiction. However, further research is necessary to establish a clearer understanding of AKAP5's role in these conditions.
Yes, the expression of AKAP5 can be regulated during development and in response to different physiological conditions or stimuli. It is known to be developmentally regulated in the brain, with changes in its expression levels during critical periods of synaptic maturation and plasticity.
While AKAP5's functions have been extensively studied in neurons, recent research suggests potential roles in other cell types. For example, AKAP5 has been implicated in cardiac function, where it regulates the localization and activity of ion channels and receptors. However, further investigation is needed to fully understand AKAP5's functions outside of the nervous system.
Yes, AKAP5 is important for synaptic plasticity, which is the ability of synapses to strengthen or weaken in response to neuronal activity. It anchors PKA and other signaling molecules to postsynaptic densities, where they modulate neurotransmitter receptor function, synaptic strength, and long-term potentiation (LTP) or long-term depression (LTD) processes associated with synaptic plasticity.
AKAP5 is widely expressed in various tissues, including the brain, heart, and skeletal muscle. It is especially abundant in neurons, where it is involved in regulating neuronal signaling and synaptic plasticity.
AKAP5 can be regulated through multiple mechanisms. One important regulatory mechanism involves phosphorylation, where various kinases can target AKAP5 and modulate its activity or binding affinity for interacting proteins. Additionally, intracellular signaling pathways, calcium levels, and changes in subcellular localization can influence AKAP5 function and its interactions with other molecules.
Due to its vital role in regulating various cellular processes, including neuronal function, AKAP5 has been considered as a potential therapeutic target. Developing drugs or compounds that selectively modulate AKAP5 interactions or disrupt specific protein-protein interactions involving AKAP5 could provide a means to regulate neuronal signaling and potentially treat certain neurological disorders.
Customer Reviews (4)
Write a reviewThe AKAP5 protein stands as a pinnacle of quality, demonstrating exceptional purity and functionality that elegantly fulfills my experimental requirements.
Beyond the protein's outstanding attributes, the manufacturer's commitment to delivering excellent technical support is truly remarkable.
By utilizing the AKAP5 protein, I can confidently delve into my experiments, knowing that it will provide unparalleled precision and effectiveness.
Its superior composition ensures utmost reliability and accuracy in achieving my research objectives.
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