Recombinant Mouse Akap5 Protein, His&GST-tagged

• Cat.No.: Akap5-110M
• Product Overview: Recombinant Mouse Akap5 Protein (Met1-Gln161) with N-His&GST tag was expressed in E. coli.

Specification

• Description: Enables adenylate cyclase binding activity. Involved in clustering of voltage-gated calcium channels; clustering of voltage-gated potassium channels; and receptor clustering. Acts upstream of or within amylase secretion. Located in cytoskeleton and plasma membrane. Is expressed in brain. Orthologous to human AKAP5 (A-kinase anchoring protein 5).
• Source: E. coli
• Species: Mouse
• Tag: N-His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 47.9 kDa
  Accurate Molecular Mass: 63 kDa
• Protein length: Met1-Gln161
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Akap5 A kinase (PRKA) anchor protein 5 [ Mus musculus (house mouse) ]
• Official Symbol: Akap5
• Synonyms: AKAP5; A kinase (PRKA) anchor protein 5; A-kinase anchor protein 5; A-kinase anchor protein 150 kDa; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein; P150; Gm258; AKAP-5; AKAP150; AKAP 150; BB098886; 3526401B18Rik
• Gene ID: 238276
• mRNA Refseq: NM_001101471
• Protein Refseq: NP_001094941
• UniProt ID: D3YVF0

Reviews

03/31/2022

The AKAP5 protein stands as a pinnacle of quality, demonstrating exceptional purity and functionality that elegantly fulfills my experimental requirements.

11/12/2020

Beyond the protein's outstanding attributes, the manufacturer's commitment to delivering excellent technical support is truly remarkable.

10/20/2020

By utilizing the AKAP5 protein, I can confidently delve into my experiments, knowing that it will provide unparalleled precision and effectiveness.

02/04/2018

Its superior composition ensures utmost reliability and accuracy in achieving my research objectives.

Q&As

Can AKAP5 be used as a diagnostic marker for any diseases?

At present, AKAP5 is not established as a specific diagnostic marker for any diseases. However, ongoing research may uncover its potential diagnostic utility in certain conditions where AKAP5 dysregulation or altered expression has been observed.

What are the structural domains of AKAP5?

AKAP5 contains multiple structural domains that contribute to its function. It has a long N-terminal region rich in potential protein-protein interaction sites, including amphipathic helices and an isoleucine-arginine motif. It also has a central domain that binds to the regulatory subunits of PKA and a C-terminal domain responsible for anchoring AKAP5 to various cellular structures.

Are there any other proteins or molecules that interact with AKAP5?

Apart from PKA, AKAP5 interacts with multiple other molecules involved in signaling pathways. It can bind and recruit various enzymes, ion channels, receptors, and cytoskeletal proteins to specific subcellular locations. Some notable interacting partners of AKAP5 include phosphodiesterases, protein phosphatase 2B (calcineurin), NMDA receptors, L-type calcium channels, and adenylyl cyclases.

Can AKAP5 interact with multiple isoforms of PKA?

Yes, AKAP5 can interact with various isoforms of PKA by binding to their regulatory subunits. This allows for selective targeting of different PKA holoenzymes to specific subcellular locations.

Are there any drugs or compounds that specifically target AKAP5?

Currently, there are no specific drugs or compounds that target AKAP5 directly. However, researchers are actively investigating the development of small molecules or peptides that can selectively disrupt AKAP5 interactions or modulate its functions.

How is AKAP5 involved in calcium signaling?

AKAP5 plays a role in calcium signaling by anchoring PKA and calcium-related enzymes or channels. It is involved in regulating calcium-induced release of neurotransmitters and modulating NMDA receptor-mediated calcium influx.

Are there any AKAP5 knockout (KO) animal models?

Yes, AKAP5 knockout animal models have been generated to study its functions. These models have provided valuable insights into the role of AKAP5 in synaptic transmission, synaptic plasticity, and behavior.

Can AKAP5 be phosphorylated?

Yes, AKAP5 can be phosphorylated by various kinases, including PKA itself. Phosphorylation of AKAP5 can regulate its localization, protein-protein interactions, and activity.

Does genetic variation in AKAP5 influence disease susceptibility?

Some studies have identified genetic variations in AKAP5 that are associated with increased susceptibility to certain psychiatric disorders, including schizophrenia and bipolar disorder. However, more research is needed to fully understand the impact of these genetic variations on disease development and progression.

How does AKAP5 interact with PKA?

AKAP5 binds to the regulatory subunits of PKA, bringing the enzyme into close proximity with its substrate molecules. This interaction allows PKA to phosphorylate specific target proteins and regulate their function within the localized signaling microdomain.

Is AKAP5 associated with any diseases or disorders?

Some studies highlight the involvement of AKAP5 in specific diseases and disorders. For instance, alterations in AKAP5 localization and function have been implicated in schizophrenia, bipolar disorder, and alcohol addiction. However, further research is necessary to establish a clearer understanding of AKAP5's role in these conditions.

Is AKAP5 expression regulated during development?

Yes, the expression of AKAP5 can be regulated during development and in response to different physiological conditions or stimuli. It is known to be developmentally regulated in the brain, with changes in its expression levels during critical periods of synaptic maturation and plasticity.

Does AKAP5 have any roles outside of neurons?

While AKAP5's functions have been extensively studied in neurons, recent research suggests potential roles in other cell types. For example, AKAP5 has been implicated in cardiac function, where it regulates the localization and activity of ion channels and receptors. However, further investigation is needed to fully understand AKAP5's functions outside of the nervous system.

Does AKAP5 play a role in synaptic plasticity?

Yes, AKAP5 is important for synaptic plasticity, which is the ability of synapses to strengthen or weaken in response to neuronal activity. It anchors PKA and other signaling molecules to postsynaptic densities, where they modulate neurotransmitter receptor function, synaptic strength, and long-term potentiation (LTP) or long-term depression (LTD) processes associated with synaptic plasticity.

Where is AKAP5 predominantly expressed?

AKAP5 is widely expressed in various tissues, including the brain, heart, and skeletal muscle. It is especially abundant in neurons, where it is involved in regulating neuronal signaling and synaptic plasticity.

How is AKAP5 regulated in cells?

AKAP5 can be regulated through multiple mechanisms. One important regulatory mechanism involves phosphorylation, where various kinases can target AKAP5 and modulate its activity or binding affinity for interacting proteins. Additionally, intracellular signaling pathways, calcium levels, and changes in subcellular localization can influence AKAP5 function and its interactions with other molecules.

Can AKAP5 be a potential therapeutic target?

Due to its vital role in regulating various cellular processes, including neuronal function, AKAP5 has been considered as a potential therapeutic target. Developing drugs or compounds that selectively modulate AKAP5 interactions or disrupt specific protein-protein interactions involving AKAP5 could provide a means to regulate neuronal signaling and potentially treat certain neurological disorders.