Recombinant Human AMDHD2 293 Cell Lysate

• Cat.No.: AMDHD2-8884HCL

Specification

• Description: Antigen standard for amidohydrolase domain containing 2 (AMDHD2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AMDHD2 amidohydrolase domain containing 2 [ Homo sapiens ]
• Official Symbol: AMDHD2
• Synonyms: AMDHD2; amidohydrolase domain containing 2; putative N-acetylglucosamine-6-phosphate deacetylase; CGI 14; glcNAc 6-P deacetylase; amidohydrolase domain-containing protein 2; CGI-14
• Gene ID: 51005
• mRNA Refseq: NM_001145815
• Protein Refseq: NP_001139287
• UniProt ID: Q9Y303
• Chromosome Location: 16p13.3
• Pathway: Amino sugar and nucleotide sugar metabolism, organism-specific biosystem; Amino sugar and nucleotide sugar metabolism, conserved biosystem; N-acetylglucosamine degradation I, organism-specific biosystem; N-acetylglucosamine degradation II, organism-specific biosystem
• Function: N-acetylglucosamine-6-phosphate deacetylase activity; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Reviews

12/14/2021

Enhanced signal stability for prolonged imaging experiments.

11/08/2019

Low assay interference from interfering substances.

03/04/2018

Consistent detection of protein post-translational modifications.

Q&As

What are the downstream signaling pathways or cellular processes influenced by AMDHD2 activity?

AMDHD2 activity can influence downstream signaling pathways involved in cell proliferation, differentiation, or stress responses, impacting cellular metabolism, growth, or survival.

What is the subcellular localization of AMDHD2 and its significance in cellular processes?

AMDHD2 exhibits a specific subcellular localization, such as in the cytoplasm or mitochondria, which is crucial for its involvement in nucleotide metabolism or other related processes.

How does AMDHD2 participate in cellular energy metabolism and the regulation of metabolic pathways?

AMDHD2 contributes to cellular energy metabolism by influencing the utilization of nucleotide-derived metabolites in energy production pathways, such as mitochondrial respiration or ATP synthesis.

How does AMDHD2 contribute to nucleotide metabolism and what are the implications for cellular physiology?

AMDHD2 plays a role in nucleotide metabolism, contributing to the biosynthesis, salvage, or recycling of nucleotides, which are essential for DNA synthesis, RNA transcription, and cellular signaling.

Can targeting AMDHD2 activity or expression provide potential therapeutic strategies for metabolic disorders or cancer?

Modulating AMDHD2 activity or expression may hold therapeutic potential for metabolic disorders, such as nucleotide metabolism-related diseases or cancer, providing opportunities for developing targeted interventions or personalized therapies.

What is the role of AMDHD2 in DNA damage repair and genome stability maintenance?

AMDHD2 is involved in DNA damage repair mechanisms, participating in the removal or repair of damaged nucleotides or DNA lesions, thus maintaining genome stability.

What are the enzymatic activities of AMDHD2 and its substrate specificity?

AMDHD2 possesses enzymatic activities, such as phosphatase or hydrolase activity, and exhibits substrate specificity towards specific nucleotide derivatives or metabolic intermediates.

How does AMDHD2 deficiency or overexpression affect cellular proliferation, differentiation, or disease progression?

AMDHD2 deficiency or dysregulation may lead to impaired nucleotide metabolism, DNA repair defects, altered cellular proliferation, or increased susceptibility to certain diseases, including cancer.

What are the molecular interactions of AMDHD2 with other proteins and their functional consequences?

AMDHD2 interacts with other proteins involved in nucleotide metabolism, DNA repair, or cellular signaling, and these interactions modulate processes such as nucleotide synthesis, repair, or cell cycle progression.

How is AMDHD2 expression regulated under different cellular conditions or in response to metabolic cues?

AMDHD2 expression is regulated at transcriptional and post-transcriptional levels, influenced by cellular signals, metabolic cues, or growth factor signaling pathways.