Recombinant Human ANO6 Protein, His-tagged

• Cat.No.: ANO6-340H
• Product Overview: Recombinant Human ANO6(Ala744~Asn824) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: This gene encodes a multi-pass transmembrane protein that belongs to the anoctamin family. This protein is an essential component for the calcium-dependent exposure of phosphatidylserine on the cell surface. The scrambling of phospholipid occurs in various biological systems, such as when blood platelets are activated, they expose phosphatidylserine to trigger the clotting system. Mutations in this gene are associated with Scott syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 13.2kDa
• Protein length: Ala744~Asn824
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 93%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: ANO6 anoctamin 6 [ Homo sapiens ]
• Official Symbol: ANO6
• Synonyms: ANO6; anoctamin 6; TMEM16F, transmembrane protein 16F; anoctamin-6; DKFZp313M0720; transmembrane protein 16F; SCTS; BDPLT7; TMEM16F; MGC104751
• Gene ID: 196527
• mRNA Refseq: NM_001025356
• Protein Refseq: NP_001020527
• MIM: 608663
• UniProt ID: Q4KMQ2

Reviews

01/15/2023

This information is crucial for researchers in understanding the protein's behavior and interaction within their specific experimental context.

07/26/2020

The manufacturer offers exceptional technical assistance, guiding researchers through various stages of their experiments.

03/10/2020

By providing comprehensive data and resources, the manufacturer enables researchers to design and implement their trials with confidence, ensuring the desired outcomes.

09/04/2016

Its reliable bioactivity makes it an ideal choice for a diverse range of experimental applications.

Q&As

How is ANO6 protein involved in calcium-activated phospholipid scrambling?

ANO6 protein has been shown to play a role in calcium-activated phospholipid scrambling, a process in which phospholipids are redistributed between the leaflets of the cell membrane. This process is important for maintaining membrane asymmetry, regulating blood clotting, and mediating apoptotic cell clearance. ANO6 acts as a calcium-activated scramblase, facilitating the movement of phospholipids across the bilayer by creating transient pores. The exact mechanism by which ANO6 accomplishes this is not fully understood, but it is thought to involve conformational changes in the protein triggered by calcium binding. Defects in ANO6-mediated phospholipid scrambling have been associated with Scott syndrome, a rare bleeding disorder.

Can genetic manipulation of ANO6 protein be used for therapeutic purposes?

There is potential for genetic manipulation of ANO6 protein to be used for therapeutic purposes. For instance, gene therapy approaches could be explored to correct ANO6 mutations associated with Scott syndrome or other diseases. This could involve introducing corrected copies of the ANO6 gene into affected cells or tissues to restore proper ANO6 protein function.

Are there any ongoing clinical trials targeting ANO6 protein?

As of my current knowledge, there are no ongoing clinical trials specifically targeting ANO6 protein. However, research in this field is continuously evolving, and it is possible that clinical trials may be initiated in the future to evaluate the therapeutic potential of targeting ANO6 in various diseases. It is important to regularly check reputable clinical trial databases and scientific literature for the most up-to-date information on ongoing studies targeting ANO6 protein.

How is ANO6 protein linked to disease?

Dysregulation of ANO6 protein has been associated with several diseases. Mutations in the ANO6 gene have been linked to Scott syndrome, a rare bleeding disorder characterized by defective phospholipid scrambling and impaired blood clotting. ANO6 expression and activity have also been implicated in various other conditions, including cystic fibrosis, hypertension, cancer, and neuronal disorders.

What are the potential therapeutic applications of ANO6 protein?

Due to its involvement in various physiological processes and its dysregulation in certain diseases, ANO6 has emerged as a potential therapeutic target. Modulation of ANO6 activity could have implications in the treatment of disorders associated with abnormal calcium signaling, such as cystic fibrosis, asthma, or cardiovascular diseases. However, further research is needed to better understand ANO6's role in these conditions and to develop specific therapies targeting this protein.

What are the implications of ANO6 protein dysfunction?

Dysfunctional ANO6 protein has been associated with several diseases and conditions. Mutations in the ANO6 gene can lead to Scott syndrome, a bleeding disorder characterized by impaired blood coagulation. ANO6 has also been implicated in other pathological conditions such as inflammatory bowel disease, cancer, and neurodegenerative disorders; however, further research is needed to fully understand its role in these conditions.

Are there any known inhibitors or activators of ANO6 protein?

Several compounds have been identified as modulators of ANO6 protein function. Examples include the activators T16A[inh]-A01 and Eact, as well as the inhibitor CaCCinh-A01. These compounds have been used in research to study ANO6 function and may have potential therapeutic applications.

Are there any ongoing clinical trials involving ANO6 protein?

As of now, there are no registered clinical trials specifically focused on ANO6 protein. However, research on ANO6 is ongoing, and it is possible that future studies may identify potential therapeutic applications and lead to clinical trials involving ANO6 or related compounds.

Are there any specific inhibitors or activators of ANO6 protein?

There are currently no specific inhibitors or activators of ANO6 protein available for clinical use. Developing selective compounds that modulate ANO6 activity is an active area of research, as such compounds could potentially be used for therapeutic purposes.

How is ANO6 protein regulated?

ANO6 protein activity can be regulated by various factors. One important mechanism is its regulation by calcium ions. ANO6 is a calcium-activated chloride channel, meaning its activity increases in the presence of intracellular calcium. This calcium dependence allows ANO6 to participate in calcium signaling pathways and respond to cellular cues. Other regulatory mechanisms may involve post-translational modifications, protein interactions, or regulatory subunits, though the specific details of these mechanisms are still being investigated.

Can ANO6 protein be targeted for potential therapeutic interventions?

Yes, ANO6 protein has emerged as a potential therapeutic target for various diseases. For example, in the context of cystic fibrosis, ANO6 has been suggested as a potential target for pharmacological intervention to enhance chloride secretion. Modulating ANO6 function could help restore proper ion transport and alleviate symptoms in cystic fibrosis patients.

What other functions or roles does ANO6 protein have?

Besides its role in calcium-activated phospholipid scrambling, ANO6 protein is involved in several other physiological processes. It has been shown to play a role in exosome release, the secretion of small extracellular vesicles involved in intercellular communication. ANO6 has also been implicated in mediating calcium-activated chloride currents and volume-regulated anion channels in certain cell types.