Recombinant Human ANO7 293 Cell Lysate

• Cat.No.: ANO7-8844HCL

Specification

• Description: Antigen standard for anoctamin 7 (ANO7), transcript variant NGEP-L is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ANO7 anoctamin 7 [ Homo sapiens ]
• Official Symbol: ANO7
• Synonyms: ANO7; anoctamin 7; PCANAP5, TMEM16G, transmembrane protein 16G; anoctamin-7; IPCA 5; NGEP; PCANAP5L; transmembrane protein 16G; prostate cancer associated protein 5; Dresden-transmembrane protein of the prostate; DTMPP; IPCA5; D-TMPP; IPCA-5; PCANAP5; TMEM16G
• Gene ID: 50636
• mRNA Refseq: NM_001001666
• Protein Refseq: NP_001001666
• MIM: 605096
• UniProt ID: Q6IWH7
• Chromosome Location: 2q37.3
• Function: chloride channel activity; ion channel activity

Reviews

05/30/2021

ANO7 is known for its high purity and quality, ensuring accurate and reproducible results.

03/28/2021

With its well-characterized nature and availability in various formats, the ANO7 protein is an advantageous tool that aids researchers in achieving their scientific goals.

02/09/2021

The manufacturer also ensures that the ANO7 protein is well-characterized, with extensive documentation on its functional properties.

05/22/2018

One of the key advantages of working with the ANO7 protein is the comprehensive support provided by its manufacturer.

07/12/2017

Whether it's troubleshooting issues, optimizing experimental protocols, or providing detailed information about the protein, the manufacturer's support team is dedicated to helping researchers overcome any challenges they may encounter.

Q&As

Is there any known interaction partner or regulatory mechanism of ANO7 protein?

The specific interaction partners and regulatory mechanisms of ANO7 protein are not yet well characterized. Further research is needed to understand its interaction network and functional regulation.

Are there any known substrates or ligands for ANO7 protein?

The substrates and ligands for ANO7 protein are not yet well defined. Further studies are needed to determine its specific binding partners and functional interactions.

Is there any known ANO7 protein homologue in other species?

Yes, ANO7 protein has homologues in other species. In humans, it shares sequence homology with ANO1-6 proteins, and similar proteins are found in other vertebrate species as well.

What are the potential roles of ANO7 protein in disease?

ANO7 protein has been implicated in several diseases. Mutations in the ANO7 gene have been associated with the development of prostate cancer and neurodegenerative disorders like Parkinson's disease.

Where is ANO7 protein expressed?

ANO7 protein is expressed in various tissues, including the brain, heart, skeletal muscle, and gastrointestinal system.