Recombinant Human APLN 293 Cell Lysate

• Cat.No.: APLN-8792HCL

Specification

• Description: Antigen standard for apelin (APLN) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: APLN apelin [ Homo sapiens ]
• Official Symbol: APLN
• Synonyms: APLN; apelin; apelin, AGTRL1 ligand; XNPEP2; AGTRL1 ligand; APJ endogenous ligand; APEL
• Gene ID: 8862
• mRNA Refseq: NM_017413
• Protein Refseq: NP_059109
• MIM: 300297
• UniProt ID: Q9ULZ1
• Chromosome Location: Xq25
• Pathway: Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Peptide ligand-binding receptors, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by GPCR, organism-specific biosystem
• Function: hormone activity; receptor binding

Reviews

12/16/2022

Western blot experiments using this protein can obtain clearly distinguishable bands, indicating that it has high clarity and legibility.

02/10/2022

In the course of repeated experiments, the stability of this protein has remained consistent, which provides us with great convenience.

12/12/2019

I appreciate the manufacturer's pursuit of product quality, and their product repeatability is very good, which makes me more confident in the experimental results.

Q&As

Does the study of APLN involve genetic engineering technology?

Yes, genetic engineering techniques are often used to produce APLN s.

Does APLN interact with other drugs?

There may be interactions, and it is recommended to avoid concurrent use of other drugs during use, especially those that affect the cardiovascular system.

What is the action mechanism of APLN ?

APLN promotes cardiovascular protection and anti-tumor effects by binding to APJ receptors.

In what diseases might APLN have potential applications?

APLN has potential applications in the treatment of cardiovascular diseases, tumors and metabolic diseases.

In what way can APLN be administered?

APLN can be administered intravenously or subcutaneously.

What is the dose of APLN to treat cardiovascular disease?

Dosage should be determined according to the specific situation, it is recommended to consult with a professional doctor.

Does the development of APLN involve animal experiments?

In the development process, it usually goes through a series of animal experiments to verify its effectiveness and safety.

What are the side effects of APLN ?

Common side effects include injection site pain, fever, nausea, etc., but most patients tolerate them well.

What is the protective effect of APLN on cardiovascular diseases?

APLN can promote blood vessel dilation, inhibit inflammatory response, and facilitate the survival and repair of cardiomyocytes.

Has the application of APLN in tumor therapy been clinically verified?

There are currently no large-scale clinical trials to confirm its use in cancer treatment, but preliminary research results are promising.