Recombinant Human APLN, His-tagged, S tagged

• Cat.No.: APLN-3549H
• Product Overview: Apelin (APLN)

Specification

• Description: This gene encodes a peptide that functions as an endogenous ligand for the G protein coupled receptor APJ. The encoded protein is synthesized as a prepropeptide that is processed into biologically active C-terminal fragments. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1.
• Source: E. Coli
• Species: Human
• Tag: His,S
• Form: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Molecular Mass: 11.8kDa
• Protein length: 77
• Endotoxin: <1.0eu per 1ug (determined by the lal</1.0eu
• Purity: >95%
• Applications: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Notes: Small volumes of APLN recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: APLN apelin [ Homo sapiens ]
• Official Symbol: APLN
• Synonyms: APLN; apelin; apelin, AGTRL1 ligand; XNPEP2; AGTRL1 ligand; APJ endogenous ligand; APEL
• Gene ID: 8862
• mRNA Refseq: NM_017413
• Protein Refseq: NP_059109
• MIM: 300297
• UniProt ID: Q9ULZ1
• Chromosome Location: Xq25
• Pathway: Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Peptide ligand-binding
• Function: hormone activity; receptor binding

Reviews

12/16/2022

Western blot experiments using this protein can obtain clearly distinguishable bands, indicating that it has high clarity and legibility.

02/10/2022

In the course of repeated experiments, the stability of this protein has remained consistent, which provides us with great convenience.

12/12/2019

I appreciate the manufacturer's pursuit of product quality, and their product repeatability is very good, which makes me more confident in the experimental results.

Q&As

Does the study of APLN involve genetic engineering technology?

Yes, genetic engineering techniques are often used to produce APLN s.

Does APLN interact with other drugs?

There may be interactions, and it is recommended to avoid concurrent use of other drugs during use, especially those that affect the cardiovascular system.

What is the action mechanism of APLN ?

APLN promotes cardiovascular protection and anti-tumor effects by binding to APJ receptors.

In what diseases might APLN have potential applications?

APLN has potential applications in the treatment of cardiovascular diseases, tumors and metabolic diseases.

In what way can APLN be administered?

APLN can be administered intravenously or subcutaneously.

What is the dose of APLN to treat cardiovascular disease?

Dosage should be determined according to the specific situation, it is recommended to consult with a professional doctor.

Does the development of APLN involve animal experiments?

In the development process, it usually goes through a series of animal experiments to verify its effectiveness and safety.

What are the side effects of APLN ?

Common side effects include injection site pain, fever, nausea, etc., but most patients tolerate them well.

What is the protective effect of APLN on cardiovascular diseases?

APLN can promote blood vessel dilation, inhibit inflammatory response, and facilitate the survival and repair of cardiomyocytes.

Has the application of APLN in tumor therapy been clinically verified?

There are currently no large-scale clinical trials to confirm its use in cancer treatment, but preliminary research results are promising.