Recombinant Human ARPC1A protein, GST-tagged

• Cat.No.: ARPC1A-844H
• Product Overview: Human ARPC1A full-length ORF ( AAH39594, 1 a.a. - 370 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 66.44 kDa
• AA Sequence: MSLHQFLLEPITCHAWNRDRTQIAL SPNNHEVHIYKKNGSQWVKAHELKE HNGHITGIDWAPKSDRIVTCGADRN AYVWSQKDGVWKPTLVILRINRAAT FVKWSPLENKFAVGSGARLISVCYF ESENDWWVSKHIKKPIRSTVLSLDW HPNNVLLAAGSCDFKCRVFSAYIKE VDEKPASTPWGSKMPFGQLMSEFGG SGTGGWVHGVSFSASGSRLAWVSHD STVSVADASKSVQVSTLKTEFLPLL SVSFVSENSVVAAGHDCCPMLFNYD DRGCLTFVSKLDIPKQSIQRNMSAM ERFRNMDKRATTEDRNTALETLHQN SITQVSIYEVDKQDCRKFCTTVIDG AMTIWDFKTLESSIQGLRIM
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ARPC1A actin related protein 2/3 complex, subunit 1A, 41kDa [ Homo sapiens ]
• Official Symbol: ARPC1A
• Synonyms: ARPC1A; actin related protein 2/3 complex, subunit 1A, 41kDa; actin related protein 2/3 complex, subunit 1A (41 kD); actin-related protein 2/3 complex subunit 1A; actin binding protein (Schizosaccharomyces pombe sop2 like); Arc40; SOP2 like protein; SOP2Hs; SOP2L; SOP2-like protein; actin binding protein (Schizosaccharomyces pombe sop2-like)
• Gene ID: 10552
• mRNA Refseq: NM_001190996
• Protein Refseq: NP_001177925
• MIM: 604220
• UniProt ID: Q92747

Reviews

04/14/2023

Their dedicated team of experts is prompt, knowledgeable, and readily available to tackle any hurdles or concerns I may encounter.

12/13/2022

Used in protein electron microscopy structure analysis.Great.

11/11/2019

The ARPC1A protein showcases unparalleled quality, ensuring it surpasses expectations and satisfactorily addresses my experimental requirements.

Q&As

Are there any known post-translational modifications of the ARPC1A protein?

Yes, the ARPC1A protein can undergo post-translational modifications, including phosphorylation and acetylation, which may regulate its activity and interactions with other proteins. The specific effects and functional significance of these modifications are still being studied.

Can the ARPC1A protein form complexes with other protein families apart from the Arp2/3 complex?

Yes, in addition to its association with the other subunits of the Arp2/3 complex, the ARPC1A protein can interact with various actin-binding proteins and regulatory factors. For example, it can associate with WASP (Wiskott-Aldrich syndrome protein) family members, which play a crucial role in actin cytoskeleton regulation.

What are the structural features of the ARPC1A protein?

The ARPC1A protein consists of multiple domains that are involved in protein-protein interactions. It has a predicted alpha-helical coiled-coil domain near the N-terminus, which mediates interactions with other subunits of the Arp2/3 complex. The C-terminus of ARPC1A contains an actin-binding-like domain (ABD) that helps anchor the complex to newly polymerized actin filaments.

Are there any known interacting partners of the ARPC1A protein?

The ARPC1A protein interacts with other subunits of the Arp2/3 complex, such as ARPC1B, ARPC2, and ARPC5, to form the functional complex. It also interacts with various actin-binding proteins, regulatory proteins, and signaling molecules that help regulate actin polymerization and participate in specific cellular processes.

Is the ARPC1A protein expressed in all cell types?

Yes, the ARPC1A protein is expressed in most, if not all, cell types. Actin filament nucleation and branching are essential processes for various cellular functions, so the ARPC1A protein is required in a wide range of cell types and tissues.

Are there any known diseases or disorders associated with ARPC1A protein dysfunction?

Yes, mutations in the ARPC1A gene have been linked to a rare genetic disorder called ARPC1A-related intellectual disability, which is characterized by developmental delay, intellectual disability, and distinctive facial features. These mutations can disrupt the normal function of the ARPC1A protein, impairing actin filament nucleation and branching processes.

How is the ARPC1A protein regulated in cells?

The activity and expression of the ARPC1A protein can be regulated in multiple ways. It can be regulated at the transcriptional level through various transcription factors and signaling pathways. Post-translational modifications, such as phosphorylation and acetylation, can also regulate its activity. Additionally, the availability of other subunits of the Arp2/3 complex can influence the assembly and stability of the complex, thereby affecting ARPC1A function.