Recombinant Human ARPP19 Protein, GST-tagged

• Cat.No.: ARPP19-154H
• Product Overview: Recombinant Human ARPP19 fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Description: ARPP19, cAMP-regulated phosphoprotein 19, is specifically phosphorylated at Ser-62 during mitosis by MASTL, also known as the Greatwall kinase. The phos-phorylation of ARPP19 leads to inactivation of PP2A through its interaction with PR55-delta, which is essential in maintaining the activity of CDK1/cyclin B1 and promoting mitotic G2/M transition. Significant reduction of ARPP-19 was observed in brains of Alzheimer’s disease and Down syndrome. ARPP-19 was also found to contribute to tumor growth in hepatocellular carcinoma and papillary carcinoma.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol
• Molecular Mass: 46 kDa
• Purity: > 95%
• Applications: Western Blot
• Stability: 1 year at -70 centigrade from date of shipment.
• Storage: Store product at –70centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.5 ug/ml

Gene Information

• Gene Name: ARPP19 cAMP-regulated phosphoprotein, 19kDa [ Homo sapiens(human) ]
• Official Symbol: ARPP19
• Synonyms: ARPP19; ENSAL; ARPP16; ARPP-16; ARPP-19; cAMP-regulated phosphoprotein, 19kDa; cAMP-regulated phosphoprotein 19; cyclic AMP phosphoprotein, 19 kD
• Gene ID: 10776
• mRNA Refseq: NM_006628
• Protein Refseq: NP_006619
• MIM: 605487
• UniProt ID: P56211

Reviews

01/22/2021

The knowledgeable and experienced support team is readily available to address any questions or concerns that researchers may have throughout their experimental journey.

11/10/2018

Its stability and reliability warrant its inclusion in my research protocols, leading to meaningful and conclusive results.

01/25/2017

One remarkable aspect of working with the ARPP19 protein is the excellent technical support provided by its manufacturer.

Q&As

Is the expression of ARPP19 protein regulated during development or in response to specific stimuli?

The regulation of ARPP19 expression is not extensively studied. However, some studies have suggested that its expression can be modulated in response to various signaling pathways, hormones, and stress conditions.

Are there any known interacting partners of ARPP19 protein?

Yes, ARPP19 has been found to interact with several proteins, including PP1, calcineurin, GAPDH, NudC, and NudCL2. These interactions might contribute to the modulation of PP1 activity and other cellular processes.

Can ARPP19 protein form homo- or hetero-multimers?

ARPP19 protein has been observed to form homodimers, meaning it can bind to itself and create dimers. This dimerization might play a role in its function and regulation.

Are there any known diseases or conditions associated with abnormalities in ARPP19 protein expression or function?

To date, no specific diseases or conditions have been directly linked to abnormalities in ARPP19 protein expression or function. However, further research is necessary to explore any potential associations.

Does the ARPP19 protein have any known post-translational modifications?

Yes, ARPP19 can undergo phosphorylation at specific sites, which might regulate its activity or interactions with other proteins. However, the exact functional consequences of these modifications are still under investigation.