Recombinant Human ARSB protein, His-tagged

• Cat.No.: ARSB-599H

Specification

• Product overview: Recombinant Human ARSB (P15848) aa. (Leu81~Met533) fused with His tag was produced in E. coli cells.
• Description: Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 55.2kDa; Accurate Molecular Mass: 55kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Leu81~Met533
• Purity: >80%
• Characteristic: Isoelectric point is 7.7
  .
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: ARSB arylsulfatase B [ Homo sapiens (human) ]
• Official Symbol: ARSB
• Synonyms: ARSB; arylsulfatase B; ASB; G4S; MPS6; arylsulfatase B; N-acetylgalactosamine-4-sulfatase
• Gene ID: 411
• mRNA Refseq: NM_000046.3
• Protein Refseq: NP_000037.2
• UniProt ID: P15848

Reviews

01/25/2023

the ARSB protein has proven to be a valuable asset in protein electron microscopy structure analysis.

09/09/2019

By utilizing the ARSB protein, researchers can unravel the intricate three-dimensional architecture of proteins, furthering our understanding of their function and facilitating the development of novel therapeutic interventions.

08/03/2019

The ARSB protein's superior performance in ELISA paves the way for robust and precise data analysis, providing invaluable insights into various biological processes.

Q&As

Is the ARSB protein involved in any other biological processes or diseases?

Although the primary function of the ARSB protein is related to the breakdown of GAGs, recent studies have suggested its involvement in other biological processes. It has been implicated in cellular signaling, inflammatory responses, and tissue remodeling. Additionally, ARSB mutations or dysregulation have been associated with certain cancers, neurodegenerative disorders, and metabolic disorders.

Are there any known mutations or variations in the ARSB gene?

Yes, mutations in the ARSB gene can cause mucopolysaccharidosis type VI (MPS VI). Over 170 different mutations have been identified, including insertions, deletions, and point mutations, each leading to a deficiency in ARSB enzyme activity.

What are the consequences of ARSB protein deficiency?

A deficiency in the ARSB protein leads to a rare inherited disorder called mucopolysaccharidosis type VI (MPS VI). This condition results in the accumulation of GAGs in various tissues and organs, causing various symptoms including skeletal abnormalities, cardiovascular issues, respiratory problems, and impaired vision and hearing.

How is the ARSB protein regulated in the body?

The expression of the ARSB protein is regulated by various factors, including transcription factors and signaling pathways that control gene expression. Additionally, post-translational modifications such as phosphorylation or glycosylation can affect its activity and localization within the cell.

Are there any treatments available for mucopolysaccharidosis type VI?

Yes, there are treatments available for mucopolysaccharidosis type VI. Enzyme replacement therapy (ERT) involves regular infusion of a man-made version of the ARSB enzyme to help break down the accumulated GAGs. Additionally, supportive therapies such as physical therapy, surgery, and medications are utilized to manage symptoms and complications. Gene therapy approaches are also being explored.

How is the ARSB protein related to lysosomal storage disorders?

Mutations in the ARSB gene lead to a deficiency in the ARSB enzyme, resulting in the accumulation of GAGs within lysosomes. This accumulation is characteristic of lysosomal storage disorders, including mucopolysaccharidosis type VI. These disorders occur due to impaired lysosomal function, which leads to the build-up of undigested substances and subsequent cellular dysfunction.