Recombinant Human ARSD 293 Cell Lysate

• Cat.No.: ARSD-8676HCL

Specification

• Description: Antigen standard for arylsulfatase D (ARSD), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ARSD?arylsulfatase D [?Homo sapiens?(human) ]
• Official Symbol: ARSD
• Synonyms: ARSD; arylsulfatase D; ASD; arylsulfatase D; NP_001660.2; NP_001660.2
• Gene ID: 414
• mRNA Refseq: NM_001669
• Protein Refseq: NP_001660
• MIM: 300002
• UniProt ID: P51689
• Chromosome Location: Xp22.3
• Pathway: Estrogen metabolism, organism-specific biosystem; Glycosphingolipid metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem
• Function: arylsulfatase activity; metal ion binding

Reviews

04/18/2020

The impressive performance of the ARSD protein both in ELISA assays and protein electron microscopy structure analysis makes it a highly versatile and recommended choice for scientists across various disciplines.

11/01/2017

the ARSD protein finds extensive utility in protein electron microscopy structure analysis.

02/25/2016

Its reliability and efficacy in generating high-quality data make it an indispensable tool in the quest for unraveling the mysteries of protein structure and function.

Q&As

Is the ARSD protein involved in any specific biological pathways or processes?

While the precise role of ARSD in specific biological pathways is still under investigation, it is known to participate in the breakdown of sulfated molecules within lysosomes, contributing to normal cellular metabolism and recycling of molecules.

Are there any known inhibitors or activators of the ARSD protein?

Currently, there are no specific inhibitors or activators that have been developed for the ARSD protein. However, general inhibitors or activators of sulfatase enzymes might have an effect on ARSD activity.

What are the potential consequences of impaired ARSD activity?

If the activity of ARSD is impaired, it can lead to the accumulation of sulfated molecules within lysosomes. This can disrupt normal cellular processes and potentially contribute to lysosomal storage disorders or other related disorders.

Are there any known genetic disorders associated with mutations in the ARSD gene?

Currently, there are no known genetic disorders specifically associated with mutations in the ARSD gene. However, mutations in related sulfatase genes can lead to various lysosomal storage disorders, such as multiple sulfatase deficiency (MSD).

How is the ARSD protein regulated?

The regulation of ARSD protein activity is not well understood. However, like other lysosomal enzymes, its activity may be influenced by factors such as pH and post-translational modifications.