Recombinant Human ARSD protein, His-tagged

• Cat.No.: ARSD-3278H
• Product Overview: Recombinant Human ARSD protein(242 - 593 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 242 - 593 aa
• AA Sequence: ISWYSSFGFVRRWNCILMRNHDVTE QPMVLEKTASLMLKEAVSYIERHKH GPFLLFLSLLHVHIPLVTTSAFLGK SQHGLYGDNVEEMDWLIGKVLNAIE DNGLKNSTFTYFTSDHGGHLEARDG HSQLGGWNGIYKGGKGMGGWEGGIR VPGIFHWPGVLPAGRVIGEPTSLMD VFPTVVQLVGGEVPQDRVIDGHSLV PLLQGAEARSAHEFLFHYCGQHLHA ARWHQKDSGSVWKVHYTTPQFHPEG AGACYGRGVCPCSGEGVTHHRPPLL FDLSRDPSEARPLTPDSEPLYHAVI ARVGAAVSEHRQTLSPVPQQFSMSN ILWKPWLQPCCGHFPFCSCHEDGDG TP
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Reviews

04/18/2020

The impressive performance of the ARSD protein both in ELISA assays and protein electron microscopy structure analysis makes it a highly versatile and recommended choice for scientists across various disciplines.

11/01/2017

the ARSD protein finds extensive utility in protein electron microscopy structure analysis.

02/25/2016

Its reliability and efficacy in generating high-quality data make it an indispensable tool in the quest for unraveling the mysteries of protein structure and function.

Q&As

Is the ARSD protein involved in any specific biological pathways or processes?

While the precise role of ARSD in specific biological pathways is still under investigation, it is known to participate in the breakdown of sulfated molecules within lysosomes, contributing to normal cellular metabolism and recycling of molecules.

Are there any known inhibitors or activators of the ARSD protein?

Currently, there are no specific inhibitors or activators that have been developed for the ARSD protein. However, general inhibitors or activators of sulfatase enzymes might have an effect on ARSD activity.

What are the potential consequences of impaired ARSD activity?

If the activity of ARSD is impaired, it can lead to the accumulation of sulfated molecules within lysosomes. This can disrupt normal cellular processes and potentially contribute to lysosomal storage disorders or other related disorders.

Are there any known genetic disorders associated with mutations in the ARSD gene?

Currently, there are no known genetic disorders specifically associated with mutations in the ARSD gene. However, mutations in related sulfatase genes can lead to various lysosomal storage disorders, such as multiple sulfatase deficiency (MSD).

How is the ARSD protein regulated?

The regulation of ARSD protein activity is not well understood. However, like other lysosomal enzymes, its activity may be influenced by factors such as pH and post-translational modifications.