Recombinant Human ASAH1 Protein, Myc/DDK-tagged, C13 and N15-labeled
Cat.No. : | ASAH1-3526H |
Product Overview : | ASAH1 MS Standard C13 and N15-labeled recombinant protein (NP_004306) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells. |
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Description : | This gene encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. Processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene are associated with the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy. |
Source : | HEK293 |
Species : | Human |
Tag : | Myc/DDK |
Molecular Mass : | 46.3 kDa |
AA Sequence : | MNCCIGLGEKARGSHRASYPSLSAL FTEASILGFGSFAVKAQWTEDCRKS TYPPSGPTYRGAVPWYTINLDLPPY KRWHELMLDKAPMLKVIVNSLKNMI NTFVPSGKVMQVVDEKLPGLLGNFP GPFEEEMKGIAAVTDIPLGEIISFN IFYELFTICTSIVAEDKKGHLIHGR NMDFGVFLGWNINNDTWVITEQLKP LTVNLDFQRNNKTVFKASSFAGYVG MLTGFKPGLFSLTLNERFSINGGYL GILEWILGKKDAMWIGFLTRTVLEN STSYEEAKNLLTKTKILAPAYFILG GNQSGEGCVITRDRKESLDVYELDA KQGRWYVVQTNYDRWKHPFFLDDRR TPAKMCLNRTSQENISFETMYDVLS TKPVLNKLTVYTTLIDVTKGQFETY LRDCPDPCIGWTRTRPLEQKLISEE DLAANDILDYKDDDDKV |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : | Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : | 50 μg/mL as determined by BCA |
Storage Buffer : | 100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
SDS-PAGE: |
Gene Name : | ASAH1 N-acylsphingosine amidohydrolase 1 [ Homo sapiens (human) ] |
Official Symbol : | ASAH1 |
Synonyms : | ASAH1; N-acylsphingosine amidohydrolase (acid ceramidase) 1; ASAH, N acylsphingosine amidohydrolase (acid ceramidase); acid ceramidase; AC; FLJ21558; PHP32; acid CDase; acylsphingosine deacylase; putative 32 kDa heart protein; PHP; ASAH; ACDase; FLJ22079; |
Gene ID : | 427 |
mRNA Refseq : | NM_004315 |
Protein Refseq : | NP_004306 |
MIM : | 613468 |
UniProt ID : | Q13510 |
Products Types
◆ Recombinant Protein | ||
Asah1-655M | Recombinant Mouse Asah1 Protein, MYC/DDK-tagged | +Inquiry |
ASAH1-65C | Recombinant Cynomolgus Monkey ASAH1 Protein, His (Fc)-Avi-tagged | +Inquiry |
ASAH1-2093M | Recombinant Mouse ASAH1 Protein (19-141 aa), His-GST-Myc-tagged | +Inquiry |
ASAH1-524H | Recombinant Human ASAH1 Protein, His-tagged | +Inquiry |
ASAH1-1134H | Recombinant Human ASAH1 Protein, His-SUMO-tagged | +Inquiry |
◆ Lysates | ||
ASAH1-8670HCL | Recombinant Human ASAH1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (8)
Ask a questionGiven the involvement of ASAH1 in various diseases, including cancer, modulating its activity or developing ASAH1 inhibitors holds promise as potential therapeutic strategies.
Yes, alterations in ASAH1 gene expression or activity can impact cellular differentiation processes, as ceramide metabolism is crucial for normal development and tissue homeostasis.
Yes, mutations or deficiencies in the ASAH1 gene are associated with Farber disease, a rare inherited disorder characterized by the accumulation of ceramide in tissues throughout the body.
ASAH1 deficiency, as seen in Farber disease, is a genetic disorder. Prevention involves genetic counseling for at-risk individuals and prenatal testing during pregnancy.
Currently, ASAH1 deficiency is not included in routine newborn screening panels. However, advancements in testing methods may lead to its inclusion in the future.
Besides its role in ceramide metabolism, the ASAH1 protein can affect immune response, inflammation, and cellular stress responses.
Dysfunction of the ASAH1 protein impairs the breakdown of ceramide, leading to its accumulation in various tissues and disrupting cellular homeostasis.
Yes, the ASAH1 protein can modulate signaling pathways like the sphingosine-1-phosphate pathway, which influences various cellular processes such as migration, inflammation, and angiogenesis.
Customer Reviews (3)
Write a reviewSuch dedicated support makes me feel confident in my choice of using the ASAH1 protein for my experiments.
The manufacturer's commitment to excellence is truly commendable, especially when it comes to their outstanding technical support.
The ASAH1 Protein is a protein of exceptional quality that surpasses my expectations and fulfills all my experimental requirements.
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