Recombinant Human ATP2A2 293 Cell Lysate

• Cat.No.: ATP2A2-8609HCL

Specification

• Description: Antigen standard for ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (ATP2A2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ATP2A2?ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 [?Homo sapiens?(human) ]
• Official Symbol: ATP2A2
• Synonyms: ATP2A2; ATPase, Ca++ transporting, cardiac muscle, slow twitch 2; DD; DAR; ATP2B; SERCA2; sarcoplasmic/endoplasmic reticulum calcium ATPase 2; calcium pump 2; SR Ca(2+)-ATPase 2; cardiac Ca2+ ATPase; endoplasmic reticulum class 1/2 Ca(2+) ATPase; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; NP_001672.1; EC 3.6.3.8; NP_733765.1
• Gene ID: 488
• mRNA Refseq: NM_170665
• Protein Refseq: NP_733765
• MIM: 108740
• UniProt ID: P16615
• Chromosome Location: 12q24.11
• Pathway: Adrenergic signaling in cardiomyocytes, organism-specific biosystem; Alzheimer's disease, conserved biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), conserved biosystem; Cardiac muscle contraction, organism-specific biosystem
• Function: ATP binding; calcium-transporting ATPase activity; calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential

Reviews

08/09/2020

Because ATP2A2 has a short half-life and high clearance, it better prevents drug accumulation in the body, thereby reducing the risk of side effects caused by long-term use.

07/28/2019

The production process of ATP2A2 focuses on harmonious coexistence with nature, realizes zero pollution in the production process, and brings a positive impact on the environment.

03/25/2019

It can maintain stable performance even in extremely harsh environments, which is a rare quality.

Q&As

Are ATP2A2 recombinant protein mutations associated with human nervous system related diseases?

Mutations in the recombinant ATP2A2 protein are associated with several nervous system related diseases, such as neurodegenerative diseases and neurodysregulation.

What are the structural features of the recombinant ATP2A2 protein?

ATP2A2 protein is a calcium pump protein containing multiple membrane channels and ATP-binding sites for the regulation of intracellular calcium homeostasis.

Is the expression and function of recombinant ATP2A2 protein regulated in the nervous system?

Yes, the expression and function of ATP2A2 recombinant protein in the nervous system are regulated by complex mechanisms, including endogenous signaling pathways and cell-to-cell interactions.

Is there any polymorphic variation or mutation in ATP2A2 recombinant protein?

Yes, there are polymorphic variations or mutations in ATP2A2 recombinant protein, which may be related to the susceptibility and pathogenesis of nervous system related diseases.

What is the function of ATP2A2 recombinant protein in the nervous system?

The recombinant ATP2A2 protein is responsible for regulating calcium homeostasis and maintaining nerve cell communication in the nervous system.

Does the recombinant ATP2A2 protein interact with other proteins?

The recombinant ATP2A2 protein interacts with other membrane proteins, such as calcium channels and transporters, to regulate the function of nerve cells.