Recombinant Human ATP2A2 Protein, His-tagged

• Cat.No.: ATP2A2-266H
• Product Overview: Recombinant Human ATP2A2 Protein (Ile315-Met756) with N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of the skeletal muscle. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Other types of mutations in this gene have been associated with various forms of muscular dystrophies. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 51.9 kDa
  Accurate Molecular Mass: 56 kDa
• Protein length: Ile315-Met756
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: ATP2A2 ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 [ Homo sapiens (human) ]
• Official Symbol: ATP2A2
• Synonyms: ATP2A2; ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2; DD; DAR; ATP2B; SERCA2; sarcoplasmic/endoplasmic reticulum calcium ATPase 2; ATPase Ca++ transporting cardiac muscle slow twitch 2; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; SR Ca(2+)-ATPase 2; calcium pump 2; cardiac Ca2+ ATPase; endoplasmic reticulum class 1/2 Ca(2+) ATPase; EC 7.2.2.10
• Gene ID: 488
• mRNA Refseq: NM_001681
• Protein Refseq: NP_001672
• MIM: 108740
• UniProt ID: P16615

Reviews

08/09/2020

Because ATP2A2 has a short half-life and high clearance, it better prevents drug accumulation in the body, thereby reducing the risk of side effects caused by long-term use.

07/28/2019

The production process of ATP2A2 focuses on harmonious coexistence with nature, realizes zero pollution in the production process, and brings a positive impact on the environment.

03/25/2019

It can maintain stable performance even in extremely harsh environments, which is a rare quality.

Q&As

Are ATP2A2 recombinant protein mutations associated with human nervous system related diseases?

Mutations in the recombinant ATP2A2 protein are associated with several nervous system related diseases, such as neurodegenerative diseases and neurodysregulation.

What are the structural features of the recombinant ATP2A2 protein?

ATP2A2 protein is a calcium pump protein containing multiple membrane channels and ATP-binding sites for the regulation of intracellular calcium homeostasis.

Is the expression and function of recombinant ATP2A2 protein regulated in the nervous system?

Yes, the expression and function of ATP2A2 recombinant protein in the nervous system are regulated by complex mechanisms, including endogenous signaling pathways and cell-to-cell interactions.

Is there any polymorphic variation or mutation in ATP2A2 recombinant protein?

Yes, there are polymorphic variations or mutations in ATP2A2 recombinant protein, which may be related to the susceptibility and pathogenesis of nervous system related diseases.

What is the function of ATP2A2 recombinant protein in the nervous system?

The recombinant ATP2A2 protein is responsible for regulating calcium homeostasis and maintaining nerve cell communication in the nervous system.

Does the recombinant ATP2A2 protein interact with other proteins?

The recombinant ATP2A2 protein interacts with other membrane proteins, such as calcium channels and transporters, to regulate the function of nerve cells.