Recombinant Human Cyclin-Dependent Kinase 6, His-tagged

• Cat.No.: CDK6-1002H
• Product Overview: Recombinant full-length human CDK6 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Cat. No.: CDK6-1002H
• Description: CDK6 is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors. The CDK6 kinase activity is detected in mid-G1 phase of the cell cycle and is responsible for the phosphorylation and regulation of the activity of tumor suppressor protein Rb. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivities in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 39 kDa
• Expression System: Sf9 insect cells using baculovirus
• Species: Human
• Form: Recombinant protein stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25 % glycerol.
• Purity: > 90 %
• Concentration: 0.2 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: B Cell Receptor Signaling Pathway; C-MYB transcription factor network; Cell Cycle; Chronic myeloid leukemia; Coregulation of Androgen receptor activity; Cyclin D associated events in G1; G1 Phase; G1 to S cell cycle control; Glioma; IL2 signaling events mediated by STAT5; Measles; Melanoma; Mitotic G1-G1/S phases; Non-small cell lung cancer; Pancreatic cancer

Gene Information

• Gene Name: CDK6 cyclin-dependent kinase 6 [ Homo sapiens ]
• Official Symbol: CDK6
• Synonyms: CDK6; cyclin-dependent kinase 6; PLSTIRE; MGC59692; OTTHUMP00000162478; OTTHUMP00000208823; cell division protein kinase 6; serine/threonine-protein kinase PLSTIRE; Cyclin-dependent kinase 6; EC 2.7.11.22
• Gene ID: 1021
• mRNA Refseq: NM_001145306
• Protein Refseq: NP_001138778
• MIM: 603368
• UniProt ID: Q00534
• Chromosome Location: 7q21-q22
• Function: ATP binding; cyclin binding; cyclin-dependent protein kinase activity; nucleotide binding; protein binding

Reviews

07/06/2023

The results obtained after analysis by SDS-PAGE or Western blot were good.

03/14/2022

It has a wide range of applications and can be used in various biological experiments.

10/26/2020

The purity of CDK6 is good, and the experimental results obtained are very accurate.

Q&As

How is the expression level of CDK6 regulated?

The expression level of CDK6 is regulated by a variety of factors, including transcription factors, epigenetic modifications, and signal transduction pathways. These factors can affect the transcription and translation processes of the CDK6 gene, thereby regulating its expression level in cells.

What are the challenges in the development of CDK6 inhibitors?

There are several challenges in the development of CDK6 inhibitors, including issues such as inhibitor selectivity, drug resistance, and drug delivery. In addition, since CDK6 also has a certain function in normal cells, the side effects of inhibitors are also a factor to consider.

Does CDK6 have a role in stem cell biology?

It plays an important role in stem cell biology. It is involved in the process of self-renewal and differentiation of stem cells, as well as in determining the fate of stem cells. By regulating the activity of CDK6, it is possible to influence the proliferation and fate selection of stem cells.

What are the clinical applications of CDK6 inhibitors?

CDK6 inhibitors have been developed as potential anti-cancer drugs and have shown some efficacy in clinical trials. They block the progression of the cell cycle by inhibiting the activity of CDK6, thereby inhibiting the proliferation of cancer cells.

How is CDK6 activity regulated?

The activity of CDK6 is regulated at multiple levels, including binding to other cyclins and inhibitors, phosphorylation and dephosphorylation events, and transcriptional and post-translational modifications.

Does CDK6 function differently in different types of cancer?

The role of CDK6 in different types of cancer may vary. It may act as a promoter in some cancers and as a suppressant in others. This difference can be related to factors such as the type, stage, and genetic background of the cancer.