Active Recombinant Human DCXR protein, His-tagged

• Cat.No.: DCXR-128H
• Product Overview: Recombinant Human DCXR(1-244aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: Dicarbonyl/L-xylulose reductase, also known as DCXR, is an enzyme responsible for the metabolism of xylulose, converting it into xylitol. DCXR was expressed at low levels and was localized predominantly in the cytoplasmic membrane. In contrast, in virtually all grades of early-stage prostate cancer and in all chemohormonally treated cases, DCXR was strikingly overexpressed and was localized predominantly in the cytoplasm and nucleus.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 50mM NaCl
• Bio-activity: Specific activity is > 1,800 pmol/min/ug and is defined as the amount of enzyme that oxidize 1pmole of xylitol to L-xylulose per minute at pH 10.0 at 37°C.
• Molecular Mass: 28 kDa (264aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MELFLAGRRV LVTGAGKGIG RGTVQALHAT GARVVAVSRT QADLDSLVRE CPGIEPVCVD LGDWEATERA
  LGSVGPVDLL VNNAAVALLQ PFLEVTKEAF DRSFEVNLRA VIQVSQIVAR GLIARGVPGA IVNVSSQCSQ RAVTNHSVYC STKGALDMLT
  KVMALELGPH KIRVNAVNPT VVMTSMGQAT WSDPHKAKTM LNRIPLGKFA EVEHVVNAIL FLLSDRSGMT TGSTLPVEGG FWAC
• Purity: > 95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: DCXR dicarbonyl/L-xylulose reductase [ Homo sapiens ]
• Official Symbol: DCXR
• Synonyms: DCXR; dicarbonyl/L-xylulose reductase; L-xylulose reductase; DCR; KIDCR; SDR20C1; short chain dehydrogenase/reductase family 20C; member 1; carbonyl reductase 2; carbonyl reductase II; sperm surface protein P34H; kidney dicarbonyl reductase; short chain dehydrogenase/reductase family 20C, member 1; XR; HCR2; P34H; HCRII
• Gene ID: 51181
• mRNA Refseq: NM_016286
• Protein Refseq: NP_057370
• MIM: 608347
• UniProt ID: Q7Z4W1
• Chromosome Location: 17q25.3
• Pathway: D-glucuronate degradation I, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Pentose and glucuronate interconversions, organism-specific biosystem; Pentose and glucuronate interconversions, conserved biosystem
• Function: L-xylulose reductase (NADP+) activity; nucleotide binding; oxidoreductase activity, acting on CH-OH group of donors; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor

Reviews

01/25/2022

The staff were enthusiastic and proactive in communicating with me and solving many technical problems in a timely manner.

03/08/2021

The proteins purchased from your company are of very high quality and are suitable for WB experiments.

11/21/2020

DCXR proteins purchased are as advertised and the quality of the product is guaranteed.

Q&As

What are the effects of inhibiting DXCR expression?

Inhibition of DCXR protein expression is associated with male infertility.

Where is the DCXR positioned?

DCXR is a sperm surface protein localized to the sperm acrosome site.

What is the effect of ureaplasma urealyticum infection on DCXR expression?

Ureaplasma urealyticum infection can cause epididymal dysfunction leading to decreased expression of the sperm protein DCXR.

What role does the DXCR play?

DXCR has L-xylulose reductase and bicarbonyl reductase activities that kill bacteria in epididymal fluid.

What is the effect of DCXR expression on sperm viability?

DCXR can remove certain toxic substances and protect spermatozoa to a certain extent and promote sperm maturation and reduce sperm apoptosis.