Recombinant Human DDX4 protein, His-tagged

• Cat.No.: DDX4-182H
• Product Overview: Recombinant Human DDX4 protein(NP_001136021)(340-690 aa), fused with His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.). 5 % trehalose and 5 % mannitol are added as protectants before lyophilization.
• Protein length: 340-690 aa
• AA Sequence: TRELVNKIYLEARKFSFGTCVRAVV IYGGTQLGHSIRQIVQGCNILCATP GRLMDIIGKEKIGLKQIKYLVLDEA DRMLDMGFGPEMKKLISCPGMPSKE QRQTLMFSATFPEEIQRLAAEFLKS NYLFVAVGQVGGACRDVQQTVLQVG QFSKREKLVEILRNIGDERTMVFVE TKKKADFIATFLCQEKISTTSIHGD REQREREQALGDFRFGKCPVLVATS VAARGLDIENVQHVINFDLPSTIDE YVHRIGRTGRCGNTGRAISFFDLES DNHLAQPLVKVLTDAQQDVPAWLEE IAFSTYIPGFSGSTRGNVFASVDTR KGKSTLNTAGFSSSQAPNPVDDESW D
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: DDX4 DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 [ Homo sapiens ]
• Official Symbol: DDX4
• Synonyms: DDX4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 4; probable ATP-dependent RNA helicase DDX4; VASA; vasa homolog; DEAD box protein 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; MGC111074
• Gene ID: 54514
• mRNA Refseq: NM_001142549
• Protein Refseq: NP_001136021
• MIM: 605281
• UniProt ID: Q9NQI0

Reviews

08/19/2019

Their prompt assistance and willingness to go above and beyond to ensure my success have been immensely valuable.

05/09/2018

This protein has proven to be an invaluable asset in my experiments, enabling accurate and precise measurements with exceptional sensitivity.

10/24/2017

Its purity, stability, and consistency are unparalleled, ensuring reliable and reproducible results in my research.

Q&As

How is DDX4 protein expression used in clinical diagnosis?

DDX4 protein expression is used as a biomarker for germ cell development and certain reproductive diseases, aiding in diagnosis and prognosis.

Does DDX4 protein have a role in the development of male and female gametes?

Yes, DDX4 protein plays an essential role in the development of both male and female gametes, making it a crucial factor in reproductive health.

How does DDX4 protein influence oocyte and embryo development?

DDX4 protein is involved in the regulation of oocyte and embryo development, and its dysregulation can impact fertility and reproductive outcomes.

How is DDX4 protein expression assessed in clinical settings?

DDX4 protein expression can be assessed using immunohistochemistry, western blotting, and other molecular techniques in clinical settings.

Are there any known genetic mutations affecting DDX4 protein associated with clinical conditions?

Yes, genetic mutations in DDX4 protein have been linked to certain forms of infertility and germ cell tumors.