Recombinant Human DNMT3A 293 Cell Lysate

• Cat.No.: DNMT3A-6855HCL

Specification

• Description: Antigen standard for DNA (cytosine-5-)-methyltransferase 3 alpha (DNMT3A), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: DNMT3A DNA (cytosine-5-)-methyltransferase 3 alpha [ Homo sapiens ]
• Official Symbol: DNMT3A
• Synonyms: DNMT3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA (cytosine-5)-methyltransferase 3A; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2; DNMT3A2; M.HsaIIIA
• Gene ID: 1788
• mRNA Refseq: NM_022552
• Protein Refseq: NP_072046
• MIM: 602769
• UniProt ID: Q9Y6K1
• Chromosome Location: 2p23
• Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Methionine degradation, organism-specific biosystem; Methionine degradation, conserved biosystem; One Carbon Metabolism, organism-specific biosystem; Validated targets of C-MYC transcriptional repression, organism-specific biosystem
• Function: DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; DNA binding; chromatin binding; metal ion binding; methyltransferase activity; protein binding; transferase activity; unmethylated CpG binding

Reviews

11/17/2022

Its exceptional characteristics, coupled with the manufacturer's commitment to customer satisfaction, ensure that I can pursue my scientific investigations with confidence and success.

09/07/2022

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08/20/2017

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Q&As

Can DNMT3A protein be used as a biomarker for cancer diagnosis?

DNMT3A mutations can serve as biomarkers for certain cancers, aiding in the diagnosis and classification of hematologic malignancies.

How does DNMT3A impact normal cellular function?

DNMT3A is essential for normal DNA methylation patterns during development and differentiation, influencing gene expression and cellular function.

Can DNMT3A-targeted therapies have off-target effects?

Like many targeted therapies, DNMT3A-targeted treatments may have off-target effects, and their specificity is an area of active research.

Are there diagnostic tests for detecting DNMT3A mutations?

Yes, molecular diagnostic tests, such as DNA sequencing, can identify DNMT3A mutations in cancer cells.

How does DNMT3A contribute to epigenetic changes in cancer cells?

DNMT3A mutations lead to abnormal DNA methylation patterns, contributing to altered gene expression and promoting cancer development.