Recombinant Mouse Dnmt3a Protein, Myc/DDK-tagged

• Cat.No.: Dnmt3a-2621M
• Product Overview: Purified recombinant protein of mouse full-length DNA methyltransferase 3A (Dnmt3a), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene causes developmental defects in multiple different organ systems. There is a pseudogene for this gene located on chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 102.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dnmt3a DNA methyltransferase 3A [ Mus musculus (house mouse) ]
• Official Symbol: Dnmt3a
• Synonyms: DNMT3A; DNA methyltransferase 3A; DNA (cytosine-5)-methyltransferase 3A; m.MmuIIIA; DNA MTase MmuIIIA; DNA methyltransferase MmuIIIA; W91664; AU015806
• Gene ID: 13435
• mRNA Refseq: NM_007872
• Protein Refseq: NP_031898
• UniProt ID: O88508

Reviews

11/17/2022

Its exceptional characteristics, coupled with the manufacturer's commitment to customer satisfaction, ensure that I can pursue my scientific investigations with confidence and success.

09/07/2022

Its consistent performance and reproducibility have been praised by scientists who have employed it in their studies, with many reporting outstanding results and a high level of satisfaction.

08/20/2017

This level of support becomes invaluable, allowing researchers to focus on their scientific objectives while receiving expert guidance alongside.

Q&As

Can DNMT3A protein be used as a biomarker for cancer diagnosis?

DNMT3A mutations can serve as biomarkers for certain cancers, aiding in the diagnosis and classification of hematologic malignancies.

How does DNMT3A impact normal cellular function?

DNMT3A is essential for normal DNA methylation patterns during development and differentiation, influencing gene expression and cellular function.

Can DNMT3A-targeted therapies have off-target effects?

Like many targeted therapies, DNMT3A-targeted treatments may have off-target effects, and their specificity is an area of active research.

Are there diagnostic tests for detecting DNMT3A mutations?

Yes, molecular diagnostic tests, such as DNA sequencing, can identify DNMT3A mutations in cancer cells.

How does DNMT3A contribute to epigenetic changes in cancer cells?

DNMT3A mutations lead to abnormal DNA methylation patterns, contributing to altered gene expression and promoting cancer development.