Recombinant Human DNMT3A Protein, GST/StrepII-tagged
Cat.No. : | DNMT3A-01H |
Product Overview : | Human recombinant DNMT3a (residues 214-912, C-term) was expressed in Sf9 insect cells with an N-terminal GST-tag and C-terminal StrepII tag. DNA (cytosine-5)-methyltransferase 3a (DNMT3a) is required for de novo DNA methylation and methylates at both CpG and non-CpG sites. |
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Description : | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. |
Source : | Insect cell |
Species : | Human |
Tag : | GST/StrepII |
Molecular Mass : | 107 kDa |
Protein length : | 214-912 |
Purity : | > 80% by SDS-PAGE. |
Usage : | DNMT3a (214-912) displays DNA methyltransferase activity at concentrations of 60 nM and above, 120 min. reactions, 30 centigrade, as TCA-precipitated counts in a scintillation/filter plate assay. Reaction conditions are: 50 mM Tris, pH 8, 5 mM EDTA, 10% glycerol, 10 mM BME, 0.05 mM PMSF with 1.5 μM [3H]-SAM and 10 μg/mL Lambda DNA as substrate. |
Concentration : | 1.62 μg/μL |
Storage Buffer : | 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 3 mM DTT, 0.05% Tween 20 |
Gene Name : | DNMT3A DNA methyltransferase 3 alpha [ Homo sapiens (human) ] |
Official Symbol : | DNMT3A |
Synonyms : | DNMT3A; DNA methyltransferase 3 alpha; TBRS; HESJAS; DNMT3A2; M.HsaIIIA; DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2; EC 2.1.1.37 |
Gene ID : | 1788 |
mRNA Refseq : | NM_175629 |
Protein Refseq : | NP_783328 |
MIM : | 602769 |
UniProt ID : | Q9Y6K1 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionDNMT3A mutations can serve as biomarkers for certain cancers, aiding in the diagnosis and classification of hematologic malignancies.
DNMT3A is essential for normal DNA methylation patterns during development and differentiation, influencing gene expression and cellular function.
Like many targeted therapies, DNMT3A-targeted treatments may have off-target effects, and their specificity is an area of active research.
Yes, molecular diagnostic tests, such as DNA sequencing, can identify DNMT3A mutations in cancer cells.
DNMT3A mutations lead to abnormal DNA methylation patterns, contributing to altered gene expression and promoting cancer development.
Customer Reviews (3)
Write a reviewIts exceptional characteristics, coupled with the manufacturer's commitment to customer satisfaction, ensure that I can pursue my scientific investigations with confidence and success.
Its consistent performance and reproducibility have been praised by scientists who have employed it in their studies, with many reporting outstanding results and a high level of satisfaction.
This level of support becomes invaluable, allowing researchers to focus on their scientific objectives while receiving expert guidance alongside.
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