| Product overview : |
Recombinant Human IL4 (P05112) aa. (His25~Ser153) fused with His tag was produced in HEK293F cells. |
| Description : |
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Source : |
HEK293F |
| Species : |
Human |
| Tag : |
His |
| Form : |
Freeze-dried powder |
| Molecular Mass : |
Predicted Molecular Mass: 16.6kDa; Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions. |
| Protein length : |
His25~Ser153 |
| Endotoxin : |
<1.0EU per 1ug (determined by the LAL method) |
| Purity : |
> 95% |
| Characteristic : |
Isoelectric point is 9.3. |
| Applications : |
SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling |
| Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
| Concentration : |
200ug/mL |
| Storage buffer : |
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300. |
| Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex |
