Recombinant Human Interleukin 3 (colony-stimulating factor, multiple), His-tagged
Cat.No. : | IL3-132H |
Product Overview : | Interleukin-3 Human Recombinant produced ininsect cellsis a single, glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The IL-3 CSF is fused to a C-terminal His-tag (6xHis) and purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
- Related Products
Cat. No. : | IL3-132H |
Description : | IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. |
Source : | Insect Cells. |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation : | Lyophilized from a concentrated (1mg/ml) solution in water containing 0.5X PBS pH7.4. |
Solubility : | It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Purity : | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Amino Acid Sequence : | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Pro-Thr. |
Biological Activity : | The ED50as determined by the dose-dependant stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 1 x 10 7 IU/mg. |
Protein Content : | Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard. |
Stability : | Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL3 should be stored at 4℃between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
Gene Name : | IL3 interleukin 3 (colony-stimulating factor, multiple) [ Homo sapiens ] |
Synonyms : | IL3; interleukin 3 (colony-stimulating factor, multiple); IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF; interleukin 3; mast-cell growth factor; P-cell stimulating factor; hematopoietic growth factor; multilineage-colony-stimulating factor; P-cell-stimulating factor |
Gene ID : | 3562 |
mRNA Refseq : | NM_000588 |
Protein Refseq : | NP_000579 |
MIM : | 147740 |
UniProt ID : | P08700 |
Chromosome Location : | 5q23-q31 |
Pathway : | Apoptosis; Asthma; Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Jak-STAT signaling pathway |
Function : | cytokine activity; growth factor activity; interleukin-3 receptor binding; protein binding |
Products Types
◆ Recombinant Protein | ||
IL3-168H | Active Recombinant Human IL3 Protein | +Inquiry |
Il3-7242M | Active Recombinant Mouse Il3 Protein, His-tagged | +Inquiry |
IL3-170R | Active Recombinant Rat IL-3β Protein | +Inquiry |
Il3-027I | Active Recombinant Mouse Il3 Protein (135 aa) | +Inquiry |
IL3-1035D | Recombinant Dog IL3 Protein, His-tagged | +Inquiry |
◆ Lysates | ||
IL3-607HCL | Recombinant Human IL3 cell lysate | +Inquiry |
IL3-445RCL | Recombinant Rat IL3 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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