Recombinant Human KDM4E(1-336) Protein, N-His-tagged

• Cat.No.: KDM4E-119H
• Product Overview: N-terminal 6Histagged, recombinant, amino acids 1-336, human JMJD2E, expressed in E.coli. Purified using immobilised metal affinity chromatography.

Specification

• Description: JMJD2E (Jumonji domain-containing protein 2E), also known as KDM4E, is a histone demethylase that belongs to the JmjC domain-containing protein family. Like other members of this family, JMJD2E specifically targets di- and tri-methylated lysine residues on histones H3 and H4, and catalyzes their removal, leading to changes in chromatin structure and gene expression. JMJD2E is expressed in a variety of tissues, including the brain, heart, and liver, and is involved in the regulation of various cellular processes, including differentiation, proliferation, and apoptosis. JMJD2E has been implicated in the development of several types of cancer, including breast, ovarian, and prostate cancers. Additionally, recent studies have suggested that JMJD2E is involved in the regulation of pluripotency and differentiation of embryonic stem cells. Therefore, JMJD2E has emerged as a potential target for the development of novel therapeutics for cancer, metabolic diseases, and regenerative medicine.
• Source: E. coli
• Species: Human
• Tag: His
• Protein length: 1-336 aa
• Form: 1mg/ml of enzyme in 5mM Hepes/NaOH pH 7.4, 250mM NaCl, 50% glycerol. Frozen solution
• Molecular Mass: 41.2 kDa
• AA Sequence: 1 MHHHHHHSSG VDLGTENLYF QSMKSVHSSP QNTSHTIMTF YPTMEEFADF 51 NTYVAYMESQ GAHQAGLAKV IPPKEWKARQ MYDDIEDILI ATPLQQVTSG 101 QGGVFTQYHK KKKAMRVGQY RRLANSKKYQ TPPHQNFADL EQRYWKSHPG 151 NPPIYGADIS GSLFEESTKQ WNLGHLGTIL DLLEQECGVV IEGVNTPYLY 201 FGMWKTTFAW HTEDMDLYSI NYLHFGEPKT WYVVPPEHGQ HLERLARELF 251 PDISRGCEAF LRHKVALISP TVLKENGIPF NCMTQEAGEF MVTFPYGYHA 301 GFNHGFNCAE AINFATPRWI DYGKMASQCS CGESTVTFSM DPFVRIVQPE 351 SYELWKHR
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: Stable for 1 year at -70 centigrade from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: KDM4E lysine demethylase 4E [ Homo sapiens (human) ]
• Official Symbol: KDM4E
• Synonyms: KDM4E; lysine demethylase 4E; KDM5E; JMJD2E; KDM4DL
• Gene ID: 390245
• mRNA Refseq: NM_001161630.1
• Protein Refseq: NP_001155102.1
• MIM: 616581
• UniProt ID: B2RXH2