Recombinant Human KDM4E Protein, Strep II-tagged

• Cat.No.: KDM4E-009H
• Product Overview: Recombinant Human KDM4E protein (2-337) with a N-terminal Strep II-tag was expressed in E. coli.

Specification

• Description: The protein encoded by this intronless gene is a member of a large family of histone lysine demethylases, which use oxygen and 2-oxoglutarate to demethylate di- and trimethylated lys9 of histone H3. Derepression of genes by demethylases is sometimes involved in viral infection or carcinogenesis, so inhibitors of these enzymes are desired.
• Source: E. coli
• Species: Human
• Tag: Strep II
• Molecular Mass: 40.8 kDa
• Protein length: 2-337
• Purity: ≥75% estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmol of NADH per minute at 37 centigrade in 50 mM HEPES buffer, pH 7.4, containing 50 mM sodium chloride, 1 mM ascorbic acid, 50 μM ferrous ammonium sulfate, 1 mM NAD + , 1 mM α-ketoglutarate, 0.1 μM FDH, and 125 μM H3K9me3 peptide.
• Stability: ≥ 6 months
• Storage: -80 centigrade
• Storage Buffer: 20 mM HEPES, pH 7.4, and 150 mM sodium chloride

Gene Information

• Gene Name: KDM4E lysine demethylase 4E [ Homo sapiens (human) ]
• Official Symbol: KDM4E
• Synonyms: KDM4E; lysine demethylase 4E; KDM5E; JMJD2E; KDM4DL; lysine-specific demethylase 4E; KDM4D-like protein; [histone H3]-trimethyl-L-lysine(9) demethylase 4E; lysine (K)-specific demethylase 4E; EC 1.14.11.66
• Gene ID: 390245
• mRNA Refseq: NM_001161630
• Protein Refseq: NP_001155102
• MIM: 616581
• UniProt ID: B2RXH2