Recombinant Human PUM1 protein, His-tagged

• Cat.No.: PUM1-2069H
• Product Overview: Recombinant Human PUM1 protein(825-1186 aa), fused with His tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein Length: 825-1186 aa
• AA Sequence: MPSGRSRLLEDFRNNRYPNLQLREI AGHIMEFSQDQHGSRFIQLKLERAT PAERQLVFNEILQAAYQLMVDVFGN YVIQKFFEFGSLEQKLALAERIRGH VLSLALQMYGCRVIQKALEFIPSDQ QNEMVRELDGHVLKCVKDQNGNHVV QKCIECVQPQSLQFIIDAFKGQVFA LSTHPYGCRVIQRILEHCLPDQTLP ILEELHQHTEQLVQDQYGNYVIQHV LEHGRPEDKSKIVAEIRGNVLVLSQ HKFASNVVEKCVTHASRTERAVLID EVCTMNDGPHSALYTMMKDQYANYV VQKMIDVAEPGQRKIVMHKIRPHIA TLRKYTYGKHILAKLEKYYMKNGVD LGPICGPPNGII
• Purity: 80%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: PUM1 pumilio homolog 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: PUM1
• Synonyms: PUM1; pumilio homolog 1 (Drosophila); pumilio (Drosophila) homolog 1; pumilio homolog 1; KIAA0099; PUMH1; pumilio-1; PUMH; HSPUM; PUML1
• Gene ID: 9698
• mRNA Refseq: NM_001020658
• Protein Refseq: NP_001018494
• MIM: 607204
• UniProt ID: Q14671

Reviews

08/25/2023

PUM1 performed well in the experiment and met my expectations.

02/07/2022

PUM1 is reliable, reliable and reliable.

11/14/2021

It has the expected biological activity, such as enzyme activity, receptor binding activity, etc., and performs well.

Q&As

Is there a relationship between PUM1's structure and function?

PUM1's structure determines its ability to bind to RNA and its post-transcriptional regulatory role. The study of its structure can help us understand its function and mechanism of action.

How is PUM1 expression level associated with disease?

Recently published articles indicate that PUM1 expression levels are associated with the occurrence and development of certain cancers. High expression of PUM1 may promote tumor growth and spread.

What factors should be considered in drug development for PUM1?

Drug development for PUM1 requires consideration of its specificity, safety, pharmacokinetic properties, and potential side effects. In addition, issues such as the dosage form, route of administration and design of clinical trials need to be considered.

To illustrate the specific role of PUM1 in cancer？

In cancer, PUM1 may play a role by promoting processes such as the growth, migration and invasion of tumor cells. It could be a potential target for cancer treatment.

What are the interactions of PUM1 with other proteins?

It can interact with a variety of other proteins, including transcription factors and RNA-degrading enzymes. These interactions allow it to regulate the expression levels of specific genes.

What are the treatment strategies for PUM1?

Therapeutic strategies for PUM1 may include inhibiting its expression, blocking its binding to specific RNA sequences, or regulating its associated signal transduction pathways. These methods may have potential applications in cancer treatment.