Recombinant Human RAB13 293 Cell Lysate

• Cat.No.: RAB13-2628HCL

Specification

• Description: Antigen standard for RAB13, member RAS oncogene family (RAB13) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: RAB13 RAB13, member RAS oncogene family [ Homo sapiens ]
• Official Symbol: RAB13
• Synonyms: RAB13; RAB13, member RAS oncogene family; ras-related protein Rab-13; RAS-associated protein RAB13; growth-inhibiting gene 4 protein; cell growth-inhibiting gene 4 protein; GIG4
• Gene ID: 5872
• mRNA Refseq: NM_002870
• Protein Refseq: NP_002861
• MIM: 602672
• UniProt ID: P51153
• Chromosome Location: 1q21.2
• Pathway: Tight junction, organism-specific biosystem; Tight junction, conserved biosystem
• Function: GTP binding; GTPase activity; nucleotide binding

Reviews

08/24/2022

It is very suitable for testing the rate of enzymatic reactions, and it performs very well.

12/23/2021

RAB13 has a good labeling effect and can be used for fluorescent labeling, etc.

11/25/2020

The clearance rate is high and the harm to the human body is small.

Q&As

What are the mutation types of RAB13?

The mutation types of RAB13 include point mutations, insertions, and deletions. Some of these mutations may cause dysfunction of RAB13, while others may affect its interaction with other proteins.

Who is at high risk of RAB13 mutations?

For people at high risk of RAB13 mutations, including those with cancer or other RAB13-related diseases, genetic testing can help doctors diagnose the condition more accurately and develop a corresponding treatment plan.

Can RAB13 mutations assess the impact of RAB13 mutations on disease progression?

The impact of RAB13 mutations on disease progression can be assessed through in-depth analysis of the patient's genomic data. This can help doctors better understand the patient's condition and develop more effective treatment plans.

How can bioinformatics methods analyze the impact of RAB13 mutations on disease?

Its effect on disease can be predicted by comparing the structure and sequence of mutant and wild-type proteins. This can help doctors better understand the patient's condition and develop more effective treatment plans.

How should treatment strategies for patients with RAB13 mutations be considered in clinical practice?

In clinical practice, treatment strategies for patients with RAB13 mutations need to consider the patient's specific condition, genotype, and treatment goals. Doctors can develop a personalized treatment plan based on the patient's actual situation to improve the treatment effect and maximize the patient's quality of life.

How can I detect the expression level of RAB13?

The expression level of RAB13 can be detected by immunohistochemistry, western blotting, real-time PCR, etc. These methods can detect protein or mRNA levels of RAB13 in tissues or cells to assess changes in its expression in disease.