Recombinant Mouse Rab13 Protein, Myc/DDK-tagged

• Cat.No.: Rab13-5290M
• Product Overview: Purified recombinant protein of mouse full-length RAB13, member RAS oncogene family (Rab13), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 22.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rab13 RAB13, member RAS oncogene family [ Mus musculus (house mouse) ]
• Official Symbol: Rab13
• Synonyms: RAB13; RAB13, member RAS oncogene family; ras-related protein Rab-13; 0610007N03Rik; B230212B15Rik
• Gene ID: 68328
• mRNA Refseq: NM_026677
• Protein Refseq: NP_080953
• UniProt ID: Q9DD03

Reviews

08/24/2022

It is very suitable for testing the rate of enzymatic reactions, and it performs very well.

12/23/2021

RAB13 has a good labeling effect and can be used for fluorescent labeling, etc.

11/25/2020

The clearance rate is high and the harm to the human body is small.

Q&As

What are the mutation types of RAB13?

The mutation types of RAB13 include point mutations, insertions, and deletions. Some of these mutations may cause dysfunction of RAB13, while others may affect its interaction with other proteins.

Who is at high risk of RAB13 mutations?

For people at high risk of RAB13 mutations, including those with cancer or other RAB13-related diseases, genetic testing can help doctors diagnose the condition more accurately and develop a corresponding treatment plan.

Can RAB13 mutations assess the impact of RAB13 mutations on disease progression?

The impact of RAB13 mutations on disease progression can be assessed through in-depth analysis of the patient's genomic data. This can help doctors better understand the patient's condition and develop more effective treatment plans.

How can bioinformatics methods analyze the impact of RAB13 mutations on disease?

Its effect on disease can be predicted by comparing the structure and sequence of mutant and wild-type proteins. This can help doctors better understand the patient's condition and develop more effective treatment plans.

How should treatment strategies for patients with RAB13 mutations be considered in clinical practice?

In clinical practice, treatment strategies for patients with RAB13 mutations need to consider the patient's specific condition, genotype, and treatment goals. Doctors can develop a personalized treatment plan based on the patient's actual situation to improve the treatment effect and maximize the patient's quality of life.

How can I detect the expression level of RAB13?

The expression level of RAB13 can be detected by immunohistochemistry, western blotting, real-time PCR, etc. These methods can detect protein or mRNA levels of RAB13 in tissues or cells to assess changes in its expression in disease.