Recombinant Human RBP3 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: RBP3-6254H
• Product Overview: RBP3 MS Standard C13 and N15-labeled recombinant protein (NP_002891) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 135.36 kDa
• AA Sequence: MMREWVLLMSVLLCGLAGPTHLFQP SLVLDMAKVLLDNYCFPENLLGMQE AIQQAIKSHEILSISDPQTLASVLT AGVQSSLNDPRLVISYEPSTPEPPP QVPALTSLSEEELLAWLQRGLRHEV LEGNVGYLRVDSVPGQEVLSMMGEF LVAHVWGNLMGTSALVLDLRHCTGG QVSGIPYIISYLHPGNTILHVDTIY NRPSNTTTEIWTLPQVLGERYGADK DVVVLTSSQTRGVAEDIAHILKQMR RAIVVGERTGGGALDLRKLRIGESD FFFTVPVSRSLGPLGGGSQTWEGSG VLPCVGTPAEQALEKALAILTLRSA LPGVVHCLQEVLKDYYTLVDRVPTL LQHLASMDFSTVVSEEDLVTKLNAG LQAASEDPRLLVRAIGPTETPSWPA PDAAAEDSPGVAPELPEDEAIRQAL VDSVFQVSVLPGNVGYLRFDSFADA SVLGVLAPYVLRQVWEPLQDTEHLI MDLRHNPGGPSSAVPLLLSYFQGPE AGPVHLFTTYDRRTNITQEHFSHME LPGPRYSTQRGVYLLTSHRTATAAE EFAFLMQSLGWATLVGEITAGNLLH TRTVPLLDTPEGSLALTVPVLTFID NHGEAWLGGGVVPDAIVLAEEALDK AQEVLEFHQSLGALVEGTGHLLEAH YARPEVVGQTSALLRAKLAQGAYRT AVDLESLASQLTADLQEVSGDHRLL VFHSPGELVVEEAPPPPPAVPSPEE LTYLIEALFKTEVLPGQLGYLRFDA MAELETVKAVGPQLVRLVWQQLVDT AALVIDLRYNPGSYSTAIPLLCSYF FEAEPRQHLYSVFDRATSKVTEVWT LPQVAGQRYGSHKDLYILMSHTSGS AAEAFAHTMQDLQRATVIGEPTAGG ALSVGIYQVGSSPLYASMPTQMAMS ATTGKAWDLAGVEPDITVPMSEALS IAQDIVALRAKVPTVLQTAGKLVAD NYASAELGAKMATKLSGLQSRYSRV TSEVALAEILGADLQMLSGDPHLKA AHIPENAKDRIPGIVPMQIPSPEVF EELIKFSFHTNVLEDNIGYLRFDMF GDGELLTQVSRLLVEHIWKKIMHTD AMIIDMRFNIGGPTSSIPILCSYFF DEGPPVLLDKIYSRPDDSVSELWTH AQVVGERYGSKKSMVILTSSVTAGT AEEFTYIMKRLGRALVIGEVTSGGC QPPQTYHVDDTNLYLTIPTARSVGA SDGSSWEGVGVTPHVVVPAEEALAR AKEMLQHNQLRVKRSPGLQDHLTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: RBP3 retinol binding protein 3 [ Homo sapiens (human) ]
• Official Symbol: RBP3
• Synonyms: RBP3; retinol binding protein 3, interstitial; retinol-binding protein 3; D10S64; D10S65; D10S66; interphotoreceptor retinoid-binding protein; IRBP; RBPI
• Gene ID: 5949
• mRNA Refseq: NM_002900
• Protein Refseq: NP_002891
• MIM: 180290
• UniProt ID: P10745

Reviews

06/15/2022

Gel electrophoresis consistently displayed well-defined bands, affirming the product's purity and reliability.

12/08/2018

Replicating results underscored the reliability and relevance of the product's effects.

09/07/2018

The user-friendly design of the product made it accessible to researchers with varying levels of expertise.

Q&As

How does RBP3 contribute to the efficient transport and delivery of retinoids to photoreceptor cells in the eye?

RBP3 contributes to the efficient transport and delivery of retinoids to photoreceptor cells, facilitating their uptake for visual processes.

Are there potential therapeutic strategies targeting RBP3 for vision-related disorders involving retinoid metabolism?

Potential therapeutic strategies targeting RBP3 could address vision-related disorders involving retinoid metabolism, making it a promising focus for drug development.

Are there known binding partners or receptors that interact with RBP3, influencing its role in retinoid transport and uptake?

RBP3 interacts with binding partners, including retinoids like 11-cis-retinal, influencing its role in retinoid transport and uptake.

In which ocular compartments is RBP3 predominantly localized, specifically within the retina?

RBP3 is predominantly localized within the retina, specifically in photoreceptor cells and the retinal pigment epithelium (RPE).

In what ways does RBP3 actively regulate retinoid homeostasis and participate in the visual cycle within the eye?

RBP3 actively regulates retinoid homeostasis and participates in the visual cycle, critical for maintaining optimal visual function.

What is the primary role of Retinol-Binding Protein 3 (RBP3) in supporting visual function and retinoid metabolism?

Retinol-Binding Protein 3 (RBP3) plays a crucial role in supporting visual function and regulating retinoid metabolism.

What vision-related disorders or conditions are associated with dysregulation or abnormal expression of RBP3?

Dysregulation or abnormal expression of RBP3 is associated with vision-related disorders such as retinitis pigmentosa.